We narrowed to 11,199 results for: AGA

1. pY036_ATP1A1_G3_Array

   Plasmid

   #86619

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

   Plasmid

   #149278

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Raichu-OsRac1 DN

   Plasmid

   #133263

   Purpose

   Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(T24N) (LOC4325879 Oryza sativa)
   

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 T24N

   Promoter

   Ubi

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/myc/ER-αT2ib

   Plasmid

   #110769

   Purpose

   Expresses ER intrabody against mouse and human TLR2

   Depositor

   Thomas Böldicke

   Article

   Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

   Insert

   intrabody against mouse and human TLR2
   

   Tags

   ER retention signal (SEKDEL), ER signal peptide, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCLc-U6-shHMGA2.3-PGK-dTomato

   Plasmid

   #89606

   Purpose

   Silencing Hmga2 in human cells

   Depositor

   Fernando Fierro

   Article

   Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

   Insert

   shRNA targeting HMGA2 (HMGA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

   Plasmid

   #162098

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to HA-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   HA-FLAG-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

   Plasmid

   #162099

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-ProtC

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG416GAL-EMCV_3C

   Plasmid

   #201941

   Purpose

   Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   EMCV 3C protease (EMCVgp1 )
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-puro-Halo-hMRE11(wt)

    Plasmid

    #208393

    Purpose

    To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Mutation

    H129N

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-puro-Halo-hMRE11(H129N)

    Plasmid

    #208394

    Purpose

    To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Mutation

    H129N

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL5.0 hCortKD-Flcortmut3YD EGFP

    Plasmid

    #187264

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLT 652

    Plasmid

    #59997

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    

    Expression

    Bacterial

    Mutation

    partial genomic

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAL4-DBD-CEBPa-WT

    Plasmid

    #215601

    Purpose

    For overexpression of Gal4-DBD CEBPa WT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa (CEBPA Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcBac1-Ma-sfGFP WT

    Plasmid

    #197567

    Purpose

    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Promoter

    CMV and U6/H1

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL5.0 mCherry-hCortKD-Flcortmut3YD

    Plasmid

    #187266

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL5.0 hCortKD-Flcortmut3YF mCherry

    Plasmid

    #187265

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

    Plasmid

    #74504

    Purpose

    Overexpression of CSK delta SH3 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

    Insert

    CSK (CSK Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    del-SH3 (73 to450 amino acids)

    Promoter

    CMV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. STARR-seq luciferase validation vector_SCP1_SV40

    Plasmid

    #99311

    Purpose

    Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    SV40 enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG426GAL-KUNV_NS2B-GS-NS3

    Plasmid

    #203506

    Purpose

    Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    KUNV NS2B-GS-NS3
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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