We narrowed to 22,864 results for: Sis

1. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mito-dendra2

   Plasmid

   #55796

   Purpose

   photo-activatable mitochondrial marker for expression in mammalian cells

   Depositor

   David Chan

   Article

   Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

   Insert

   mito-Dendra2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK-Myc-Smurf1

   Plasmid

   #13676

   Depositor

   Ying Zhang

   Article

   Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

   Insert

   Smurf1 (SMURF1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDsgRNA-p15

   Plasmid

   #62656

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9-CR4

   Plasmid

   #62655

   Purpose

   Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   Cas9 nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pND004

   Plasmid

   #200950

   Purpose

   MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNX24-RFP-MS2SL

   Plasmid

   #227819

   Purpose

   Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Inserts

   3xHA-RFP-24xMS2SL
   Neo resistance
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCCL-CellCycle

   Plasmid

   #132429

   Purpose

   Expressing Fucci cell cycle indicator in mammalian cells

   Depositor

   John M. Greally

   Article

   Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

   Insert

   hCdt, hGeminin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ub-G76V-GFP

   Plasmid

   #11941

   Depositor

   Nico Dantuma

   Article

   Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

   Insert

   Ub-G76V-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Promoter

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDestTol2pACryGFP

    Plasmid

    #64022

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    GFP
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    alpha-Acrystallin promoter

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLH01

    Plasmid

    #117261

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-Lp_0640-42, sapK-sappR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD-pHuji

    Plasmid

    #61555

    Purpose

    Red fluorescent protein based pH indicator in bacterial expression vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-miR21

    Plasmid

    #21114

    Depositor

    Joshua Mendell

    Article

    Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

    Insert

    miR-21 (MIR21 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSIH1-puro-control shRNA

    Plasmid

    #26597

    Purpose

    Lentiviral control shRNA vector with puromycin selection.

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-ApaLID211A-HA

    Plasmid

    #211459

    Purpose

    Express mitochondrial localized ApaLI with D211A mutation

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-D211A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D211A

    Promoter

    CMV

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-SEpHluorin

    Plasmid

    #32001

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    mCherry
    

    Use

    Tags

    Superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6a:sgRNA#1

    Plasmid

    #64245

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge