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pMJO-6 (minus T7 RNA polymerase binding site)

Plasmid

#31991

Depositor

Mark Osborn

Article

Osborn et al Hum Gene Ther. 2011 Jun 13.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pEE391

Plasmid

#149281

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Gateway Cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAd5-B6-deltaE3

Plasmid

#122559

Purpose

Modified block 6, with deletion in E3

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 27859-30803

Use

Synthetic Biology

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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E2-Crimson-FKBP(M)

Plasmid

#85002

Purpose

Expresses E2-Crimson-FKBP(M)

Depositor

Benjamin Glick

Article

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

E2-Crimson-FKBP(M)

Expression

Yeast

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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bfloGFPc1a

Plasmid

#60750

Purpose

Bacterial expression of Amphioxus GFPc1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPc1

Tags

8xHIS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
C-Ag15

Plasmid

#11868

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Tags

His and myc

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
C-Ag20(0)

Plasmid

#11866

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Tags

His and myc

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

FKBP-GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1C

Plasmid

#139470

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

stuffer

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAG504-frFAST

Plasmid

#160055

Purpose

Expresses frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-REV1-p2A-GFP-PuroR

Plasmid

#153001

Purpose

Overexpression of human REV1

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

REV1 (REV1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rhoA WT

Plasmid

#61631

Purpose

mammalian expression of rhoA

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pX330A_D10A-1x7

Plasmid

#58777

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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