169,069 results

1. pcDNA3.1(+)IRES GFP

   Plasmid

   #51406

   Purpose

   Expresses GFP from internal ribosome entry sequence, cloning component

   Depositor

   Kathleen_L Collins

   Article

   Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

   Insert

   IRES GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHM6-alphasynuclein-WT

   Plasmid

   #40824

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Tags

   6x His and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-ATG5

   Plasmid

   #13095

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5 (Atg5 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-MS2coatProtein

   Plasmid

   #103832

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   mCherry-MS2 coat protein (cp )
   

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRH-1365 LV EF1a-CD28 IRES-EGFP

   Plasmid

   #201921

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-Basigin_iso2

   Plasmid

   #221416

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   Basigin_iso2 (BSG Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRP2350

   Plasmid

   #127580

   Purpose

   Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PtetO7.1_Flagtag_8Glycine linker
   

   Use

   Synthetic Biology; Pcr based tagging

   Tags

   Flag tag, 8glycine linker

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pclbw-mitoTagRFP

   Plasmid

   #58425

   Purpose

   Mammalian expression of tagRFP targeted to the mitochondrial matrix

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mito-tagRFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   cmv-bactin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAD-sfGFP(Sp)-Pman

    Plasmid

    #133833

    Purpose

    Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(Sp)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dual-sgRNA_hU6-mU6

    Plasmid

    #154194

    Purpose

    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE NFkB-TA-LUC-UBC-GFP-W

    Plasmid

    #49343

    Purpose

    pHAGE NFkB-TA-LUC-UBC-GFP-W

    Depositor

    Darrell Kotton

    Article

    Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

    Inserts

    eGFP (eGFP )
    Luciferase
    NFkB consensus binding sequence
    TA
    UBC
    

    Use

    Lentiviral

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. p8327 pLIX YAP1 S127A

    Plasmid

    #184528

    Purpose

    Expression of YAP1 S127A

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 S127A (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    S127A

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P-2-human RNF146 full length

    Plasmid

    #132610

    Purpose

    expresses human RNF146 full length in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

    Insert

    RNF146 (RNF146 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

    Viral Prep

    #107312-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Tags

    mCherry (physically separate, not a fusion protein)

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-FLEx-ER-TurboID (AAV8)

    Viral Prep

    #160857-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-FLEx-ER-TurboID (#160857). In addition to the viral particles, you will also receive purified pAAV-FLEx-ER-TurboID plasmid DNA. CMV-driven, Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Promoter

    CAG

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-fDIO mCherry (AAV8)

    Viral Prep

    #114471-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Flp-dependent)

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only