164,868 results

1. pDONRp5-p2 GFP-NShroom3-iLID

   Plasmid

   #170976

   Purpose

   N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

   Depositor

   Miki Ebisuya

   Article

   Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

   Insert

   NShroom3 (Shroom3 Mouse)
   

   Use

   Gateway cloning

   Tags

   eGFP and iLID

   Expression

   Mutation

   From isoform 2, deleted amino acids 1389 - 1805,…

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONRp5-p2 SspB-mCherry-CShroom3

   Plasmid

   #170977

   Purpose

   C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

   Depositor

   Miki Ebisuya

   Article

   Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

   Insert

   CShroom3 (Shroom3 Mouse)
   

   Use

   Gateway cloning

   Tags

   SspB and mCherry

   Expression

   Mutation

   Isoform X2, deleted aminoacids 1 - 1388

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Bsd

   Plasmid

   #214370

   Purpose

   Cloning vector with Blasticidin

   Depositor

   Bertrand Collet

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Integrin Beta1-mPA-GFP

   Plasmid

   #186302

   Purpose

   Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

   Depositor

   Pakorn Kanchanawong

   Article

   Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

   Insert

   Integrin Beta 1 (ITGB1 Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-V5 [IPI-SV5-Pk1] (Recombinant Antibody)

   Antibody

   #218107-rAb

   Purpose

   Anti-V5 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Randall et al J Gen Virol. 1987 Nov;68 ( Pt 11):2769-8

   Recommended Applications

   Western Blot

   Reactivity

   Source Species

   Rabbit

   Isotype

   IgG1

   Trial Size

   Not available to purchase

   Available since

   May 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

6. MCS-3_Endofin_LgBiT

   Plasmid

   #182574

   Purpose

   This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

   Depositor

   Bradley McConnell

   Article

   Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

   Insert

   zinc finger FYVE-type (ZFYVE16 Human)
   

   Use

   Tags

   LgBiT (Large BiT)

   Expression

   Mammalian

   Mutation

   FYVE domain Q739-K806

   Promoter

   Herpes simplex virus (HSV)

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 MBP TEV LIC cloning vector (2M-T)

   Plasmid

   #29708

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4 HisMax-V5-GFP-RRBP1

   Plasmid

   #92150

   Purpose

   Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   RRBP1 (RRBP1 Human)
   

   Use

   Tags

   Calreticulin signal peptide, EGFP (A207K mutation…

   Expression

   Mammalian

   Mutation

   In RRBP1: N951S and T1329L. E1279 is also missing.

   Promoter

   CMV

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mVenus N1

    Plasmid

    #27793

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF.myc.ER-E2-Crimson

    Plasmid

    #38770

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Use

    Tags

    ER retention signal (KDEL), ER signal sequence (M…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2/DEST/hTfR-HA

    Plasmid

    #69610

    Purpose

    human transferrin receptor with an HA tag fused at the C-terminus

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    human transferrin receptor (TFRC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-Syn-PSD95-mCherry

    Plasmid

    #125694

    Purpose

    Exogenous expression of PSD95 (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. phND2-N174

    Plasmid

    #31822

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    NeuroD2 (NEUROD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLSLR

    Plasmid

    #51500

    Purpose

    Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. GG-EBNA-EEA-5guides-PGK-Puro

    Plasmid

    #102898

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5guides-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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