161,697 results

1. pYPQ230-RRV

   Plasmid

   #188543

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RRV
   

   Use

   CRISPR; Gateway compatible lbcas12a-rrv entry clo…

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, D156R, R182V

   Promoter

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV IRES Luciferase

   Plasmid

   #18760

   Depositor

   Scott Lowe

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT GLUT1-FLAG

   Plasmid

   #200094

   Purpose

   WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-LacI

   Plasmid

   #46394

   Purpose

   Overproduction of LacI

   Depositor

   Florian Hollfelder

   Article

   Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

   Insert

   LacI
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   silent T to C mutation at position 19

   Promoter

   arabinose promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100852

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-HA-USP11

    Plasmid

    #22566

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-BRD4

    Plasmid

    #65378

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    R1232W

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PermaPhos Kit

    Plasmid Kit

    #1000000226

    Purpose

    This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

    Depositor

    Ryan Mehl

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CXCR7-Tango

    Plasmid

    #66265

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CXCR7 (ACKR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AAV-CAG-SPOTlight-U2GCR

    Plasmid

    #164819

    Purpose

    Reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    SPOTlight-U2GCR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HsPeg10rc3

    Plasmid

    #174859

    Purpose

    Expresses recoded human Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Homo sapiens PEG10 (PEG10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV1)

    Viral Prep

    #104492-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

19. p6599 MSCV-IP N-HAonly FOSL1

    Plasmid

    #34897

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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