We narrowed to 13,934 results for: Lor

1. R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

   Plasmid

   #159687

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

   Depositor

   Dominic Esposito

   Article

   Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

   Insert

   His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcdna6.2- myc ULK1 4SA

   Plasmid

   #27631

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 4SA (Myc Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S467A, S555A, T574A, S637A. Initial M from ULK1 r…

   Promoter

   Available since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

   Plasmid

   #170197

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

   Promoter

   CMV51

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-V5-SHLD3

   Plasmid

   #114124

   Purpose

   Expresses N-terminally tagged V5-SHLD3 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. miniTol2 x EF1a_EKAREN5 + PGK-blast

   Plasmid

   #167818

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-ZNF804B

   Plasmid

   #88756

   Purpose

   Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF804B (ZNF804B Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-FOXQ1

   Plasmid

   #101628

   Purpose

   Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   FOXQ1 (FOXQ1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. peSCBE3-NG-HF1

   Plasmid

   #218164

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

   Plasmid

   #170198

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

    Plasmid

    #156411

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid

    #216732

    Purpose

    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    sgCTG lentiviral guide RNA with eGFP tag
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIN- myc ULK1 wt

    Plasmid

    #27626

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQCXIN- myc ULK1 4SA

    Plasmid

    #27628

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

14. JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

    Plasmid

    #156408

    Purpose

    pCS2 CMV expression vector driving EYFP fused human KRAS4B

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

    Plasmid

    #167827

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

    Plasmid

    #167820

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQCXIN- myc ULK1 K46I

    Plasmid

    #27627

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid

    #170196

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge