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We narrowed to 11,938 results for: emb

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Showing: 5481 - 5500 of 11938 results

  4. PF3D7_0505700-COMP-blac-flag-his

    Plasmid
    #110988
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium membrane protein (PF3D7_0505700 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110988-plasmid-map-sequence-id-224478

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  5. PF3D7_1434400-COMP-blac-flag-his

    Plasmid
    #110981
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium membrane protein (PF3D7_1434400 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110981-plasmid-map-sequence-id-224279

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  6. PF3D7_1333300-COMP-blac-flag-his

    Plasmid
    #110972
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    transmembrane protein Tmp21 homologue, putative (PF3D7_1333300 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110972-plasmid-map-sequence-id-224268

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  7. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid
    #228941
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E, N-terminus and C-terminus are deleted. BAX…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228941-plasmid-map-sequence-id-469413

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  8. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid
    #229810
    Purpose
    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Publication
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    loxp-AmCyan-stop-lox
    Use
    Tol2 based expression vector
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229810-plasmid-map-sequence-id-461094

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  10. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Publication
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

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  11. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid
    #207636
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag
    Depositor
    Cole DeForest
    Publication
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207636-plasmid-map-sequence-id-409876

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  14. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid
    #188992
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Publication
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K109R, P245A, G328S, G381V, G429D
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188992-plasmid-map-sequence-id-371645

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  16. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid
    #183605
    Purpose
    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    HA, Human PDGFRB transmembrane domain, Mouse IgGK…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183605-plasmid-map-sequence-id-361591

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  17. pSPL3-ABCC8-9-10

    Plasmid
    #135918
    Purpose
    Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants
    Depositor
    Diana Valverde Pérez
    Publication
    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    Use
    Exon trapping
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    135918-plasmid-map-sequence-id-269938

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  19. MAC-TOP3B

    Plasmid
    #172423
    Purpose
    MAC-tagged gene expression
    Depositor
    Markku Varjosalo
    Publication
    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)
    Tags
    MAC-tag
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Nov. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172423-plasmid-map-sequence-id-340224

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  20. pPICZ:mTREK-1_cryst

    Plasmid
    #133269
    Purpose
    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP
    Depositor
    Dan Minor
    Publication
    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Tags
    SNS- 3C_cleavage_site-TAAA-GFP
    Expression
    Yeast
    Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    133269-plasmid-map-sequence-id-291942

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Showing: 5481 - 5500 of 11938 results