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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5501 - 5520 of 66674 results

  2. G protein alpha-s-mCerulean

    Plasmid
    #66084
    Purpose
    G protein alpha-s internally tagged with mCerulean and EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    66084-plasmid-map-sequence-id-118926

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  3. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190169
    Purpose
    Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Insert
    EB3N-VVDfast-mClover3-iLID (MAPRE3 Synthetic, Human)
    Use
    Lentiviral
    Tags
    -VVDfast-mClover3-iLID
    Mutation
    1-189 aa from EB3
    Promoter
    TRE
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190169-plasmid-map-sequence-id-373726

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  6. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Expression
    Mammalian
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    69445-plasmid-map-sequence-id-259015

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  7. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

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  14. hNedd4-2 CS

    Plasmid
    #86041
    Purpose
    full length NeddL3 with C to S mutation with His Xpress tag
    Depositor
    Christie Thomas
    Publication
    Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
    Tags
    His and Xpress
    Expression
    Mammalian
    Mutation
    C942S mutation in HECT domain
    Promoter
    CMV
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86041-plasmid-map-sequence-id-171539

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  19. mTagRFP-T2-ER-5

    Plasmid
    #58036
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    July 31, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58036-plasmid-map-sequence-id-210339

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  20. mPlum-ER-5

    Plasmid
    #55967
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    55967-plasmid-map-sequence-id-233117

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Showing: 5501 - 5520 of 66674 results