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pPL5608_TEF1*-Cre_TRP1

Plasmid

#60931

Purpose

Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

Mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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tdTomato-H2B-C-10

Plasmid

#58102

Purpose

Localization: Nucleus/Histones, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mTurquoise-H2B-6

Plasmid

#55557

Purpose

Localization: Nucleus/Histones, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mTurquoise

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
ECFP-H2B-6

Plasmid

#55344

Purpose

Localization: Nucleus, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

ECFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-H2B-18

Plasmid

#55055

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-H2B-C-10

Plasmid

#55057

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
pICSL4723-P1

Plasmid

#86173

Purpose

LevelP cloning Vector

Depositor

Mark Youles

Type

Empty backbone

Expression

Plant

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL4723-P1

Plasmid

#86173

Purpose

LevelP cloning Vector

Depositor

Mark Youles

Type

Empty backbone

Expression

Plant

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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RHPMP126 (mOrange2-alpha Tubulin)

Plasmid

#88835

Purpose

Localization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2

Depositor

Ricardo Henriques

Insert

alpha-tubulin (TUBA1B) (TUBA1B Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-94N

Plasmid

#53281

Purpose

Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-AuroraAKD-C-7

Plasmid

#54998

Purpose

Localization: Centrosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

AuroraA KD (AURKA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Kinase Dead - I57V, K162R

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
Cx43 delta dt GFP

Plasmid

#17671

Depositor

Cecilia Lo

Insert

Cx43 (Gja1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

a.a. 234-243 deleted

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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RHPMP126 (mOrange2-alpha Tubulin)

Plasmid

#88835

Purpose

Localization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2

Depositor

Ricardo Henriques

Insert

alpha-tubulin (TUBA1B) (TUBA1B Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-94N

Plasmid

#53281

Purpose

Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-AuroraAKD-C-7

Plasmid

#54998

Purpose

Localization: Centrosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

AuroraA KD (AURKA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Kinase Dead - I57V, K162R

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only