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Ddo_slit-1

Plasmid

#107636

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

slit-1

Use

RNAi; In situ hybridization

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_ifb

Plasmid

#107635

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

ifb

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Article

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Expression

Bacterial and Yeast

Promoter

ATG41 endogenous promoter

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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DVC_p15A_AF

Plasmid

#217588

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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DVC_pBR322_FG

Plasmid

#217600

Purpose

Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Ddo_collagen

Plasmid

#107634

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

collagen

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLPT20

Plasmid

#85460

Purpose

Repressilator with integrated reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI

Use

Synthetic Biology

Tags

ssrA

Expression

Bacterial

Promoter

PLlac, PLtet, PR, and PRNAI

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB403-Lifeact-Citrine

Plasmid

#100582

Purpose

Binary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Lifeact-Citrine

Expression

Plant

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Ddo_beta-catenin-1

Plasmid

#107639

Purpose

RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

beta-catenin-1

Use

RNAi

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

nisA promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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piRFP702-N1

Plasmid

#45456

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Expression

Mammalian

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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Ddo_porcupine-1

Plasmid

#107631

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

porcupine-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_sfrp-1

Plasmid

#107637

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

sfrp-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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DVC_pBR322_AF

Plasmid

#217598

Purpose

Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFCM1

Plasmid

#64948

Purpose

source of CmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-cat-FRT

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Ddo_opsin

Plasmid

#107630

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

opsin

Use

RNAi; In situ hybridization

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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PA-GFPmt

Plasmid

#19989

Depositor

Orian Shirihai

Article

Twig et al Am J Physiol Cell Physiol. 2006 Jul . 29

Insert

GFP

Use

Lentiviral

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
Ddo_innexin-10

Plasmid

#107633

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

innexin-10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b_HCAII

Plasmid

#187110

Purpose

Expression of HCAII in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

HCAII

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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