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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,538 results for: gfp gene

Showing: 5521 - 5540 of 25538 results

  1. pCMV-eGFP-mPlum-HA

    Plasmid
    #200009
    Purpose
    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.
    Depositor
    Thomas Noll
    Article
    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:
    Insert
    eGFP, mPlum
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200009-plasmid-map-sequence-id-398431

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  4. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Use
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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  5. pJL1-sfGFP-AQNAT

    Plasmid
    #198211
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_AQNAT
    Use
    Tags
    6x His Tag and AQNAT mutated (non-permissible) gl…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198211-plasmid-map-sequence-id-393679

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  6. pJL1-sfGFP-MOOR

    Plasmid
    #198212
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_MOOR
    Use
    Tags
    6x His Tag and MOOR glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198212-plasmid-map-sequence-id-393678

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  9. pCAG2-EGFP-C-IFT139

    Plasmid
    #218738
    Purpose
    Expresses C-terminally EGFP-tagged IFT139 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT139 (TTC21B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218738-plasmid-map-sequence-id-434231

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  12. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  18. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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Showing: 5521 - 5540 of 25538 results