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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,215 results for: plasmid dna

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Showing: 5521 - 5540 of 7215 results

  1. pCB268

    Plasmid
    #53363
    Purpose
    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.
    Depositor
    Alexander Varshavsky
    Article
    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:
    Inserts
    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    Tags
    13xMyc, Flag, and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pCSN075

    Plasmid
    #166732
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166732-plasmid-map-sequence-id-332206

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  3. pcPylRS(Y384F Y306A)

    Plasmid
    #196486
    Purpose
    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    196486-plasmid-map-sequence-id-386704

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  7. MmXYRS-4xM15

    Plasmid
    #155343
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    Humanized MmXYRS/tRNAM15
    Expression
    Mammalian
    Promoter
    CMV/U6
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155343-plasmid-map-sequence-id-310521

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  8. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  13. pCK300

    Plasmid
    #87766
    Purpose
    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87766-plasmid-map-sequence-id-177280

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  14. 1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

    Plasmid
    #238894
    Purpose
    EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.
    Depositor
    Gil Westmeyer
    Article
    Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4
    Insert
    1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    238894-plasmid-map-sequence-id-476197

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Showing: 5521 - 5540 of 7215 results