We narrowed to 27,602 results for: tat

1. pET-11a HIV-1 P6

   Plasmid

   #154201

   Purpose

   Bacterial expression of Gag P6 from HIV-1 HXB2 strain

   Depositor

   Lalit Deshmukh

   Article

   Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

   Insert

   Gag P6 protein from HIV-1 clone HXB2
   

   Use

   Tags

   GB1-6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPELL-ITSN1(DH-PH) Component 1

   Plasmid

   #170491

   Purpose

   SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

   Insert

   mCherry, ITSN(N-lobe), iFKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPELL-ITSN1(DH-PH) Component 2

   Plasmid

   #170492

   Purpose

   SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

   Insert

   myc, ITSN1(C-lobe), FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dcp2 ΔH1 WD

   Plasmid

   #163580

   Purpose

   express Dcp2 ΔH1 WD at endogenous level in S.cerevisiae

   Depositor

   Michael Rosen

   Article

   Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

   Insert

   Dcp2 (DCP2 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W50A, D54A, L255A

   Promoter

   DCP2 promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

   Plasmid

   #165029

   Purpose

   Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

   Plasmid

   #165026

   Purpose

   Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

   Plasmid

   #165028

   Purpose

   Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vimentin-DiB-RM

   Plasmid

   #168783

   Purpose

   Vimentin - DiB-RM fusion protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

   Insert

   Vimentin-DiB-RM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lyn-CFP-FRB1C_no-linker

   Plasmid

   #162434

   Purpose

   for use in CIT (chemically induced trimerization) system

   Depositor

   Takanari Inoue

   Article

   Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

   Insert

   Lyn-CFP-FRB1C_no_linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dcp2 FL-H1

    Plasmid

    #163583

    Purpose

    express Dcp2 FL-H1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    The first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…

    Promoter

    DCP2 promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dcp2 ΔN

    Plasmid

    #163575

    Purpose

    express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of N-terminal domain (1-242) of Dcp2

    Promoter

    DCP2 promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dcp2 300

    Plasmid

    #163577

    Purpose

    express Dcp2 300 at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of C-terminal domian (301-970)

    Promoter

    DCP2 promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNTI743 dual-barcoded gRNA parent vector

    Plasmid

    #164915

    Purpose

    CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lyn-YFP-Clover-FRB1N

    Plasmid

    #162439

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    Lyn-YFP-Clover-FRB1N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGST-pol1-N1

    Plasmid

    #160988

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGST-pol1-N2-2A2

    Plasmid

    #160991

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F58A/D62A

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGST-pol1-N2

    Plasmid

    #160989

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGST-pol1-N1-2A2

    Plasmid

    #160990

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F58A/D62A

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJ414 - NERD-S

    Plasmid

    #136388

    Purpose

    Expresses NERD-S static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-S
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ414 - NERD-C

    Plasmid

    #136389

    Purpose

    Expresses NERD-C static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-C
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-F30L-V39I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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