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pRS313 - prp8 N1869D

Plasmid

#90975

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

N1869D

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1870N

Plasmid

#90976

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1870N

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 I1875T

Plasmid

#90977

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

I1875T

Availability

Academic Institutions and Nonprofits only

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pAV10

Plasmid

#63213

Purpose

pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pfba1p-ACO-Tgpm1

Plasmid

#127731

Purpose

Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pykt6-ACO-Tprm9

Plasmid

#127732

Purpose

Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pykt6

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Psptdh3-ACO-Tagtef1

Plasmid

#127734

Purpose

Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptdh3

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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HDAC7 Flag

Plasmid

#13824

Purpose

Mammalian expression of human histone deacetylase 7 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC7 (HDAC7 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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HDAC6 Flag

Plasmid

#13823

Purpose

Mammalian expression of human histone deacetylase 6 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC6 (HDAC6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCMVtight-UPRT-T2A-RFP-IRES-CD

Plasmid

#126677

Purpose

Doxycycline inducible expression of UPRT and CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSNR52-sgTEF1

Plasmid

#46922

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgTEF1 promoter (TEF1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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yHS1-M7A

Plasmid

#159151

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of yHS1 to A…

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-AKAR4-NES

Plasmid

#64727

Purpose

Cytosol-targeted, FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

Insert

AKAR4-NES

Tags

Cerulean, NES, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFlp-Km

Plasmid

#222334

Purpose

Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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