We narrowed to 1,213 results for: Aspa

1. pCIG-LRRC37B-HA

   Plasmid

   #220792

   Purpose

   To express the human receptor LRRC37B HA-tagged in mammalian cells together with EGFP

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

   Insert

   LRRC37B (LRRC37B Human)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST53-LRRK2-3XKD

   Plasmid

   #25049

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   "Kinase Dead" Lysine 1906 mutated to A…

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

3. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

   Plasmid

   #166693

   Purpose

   Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9-VPR
   3x Cnga1 promoter-targeting sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and U6

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129027

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW57-Cx26-IRES-GCaMP6s

   Plasmid

   #188236

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJB2 (GJB2 Human)
   

   Use

   Lentiviral

   Tags

   GCaMP6s

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHOT-P2Y2R

   Plasmid

   #159108

   Purpose

   entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

   Depositor

   Fabio Mammano

   Article

   Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

   Insert

   P2Y2R
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-N-Myc_DED/AAA USP7

   Plasmid

   #131253

   Purpose

   Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert USP7 aspart…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RANLS-LEHD-BNES

    Plasmid

    #60973

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    Caspase9 substrate sequence (LEHD) is after NLS s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129028

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST53-LRRK2-3XKD-R1441C

    Plasmid

    #25050

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDEST53-LRRK2-3XKD-Y1699C

    Plasmid

    #25051

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129036

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid

    #129053

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129029

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SB-CAG-mCherry-P2A-EIF3D-DD-IP

    Plasmid

    #236770

    Purpose

    A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528D/S529D-IRES-Pac.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    EIF3D (EIF3D Human)
    

    Use

    Sleeping beauty

    Tags

    Expression

    Mammalian

    Mutation

    changed Serine 528 to Aspartic Acid and Serine 52…

    Promoter

    CAG promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCW57-Cx26G45E-IRES-GCaMP6s

    Plasmid

    #188237

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJB2 (GJB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    p.G45E

    Promoter

    Tight TRE promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-Nix-S35D

    Plasmid

    #197563

    Purpose

    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-35. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Article

    Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 35 changed to aspartic acid

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9

    Plasmid

    #129061

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA9 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge