We narrowed to 8,425 results for: Dos

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pETMCSI-T4P_Y320A

Plasmid

#122522

Purpose

Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

Depositor

Gottfried Otting

Publication

Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

Insert

gp43 - DNA polymerase (43 Escherichia virus T4)

Expression

Bacterial

Mutation

tyrosine 320 changed to alanine

Promoter

T7 promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Publication

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLPCX-Cx43-IRES-GFP

Plasmid

#65433

Purpose

Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Publication

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTD103aiiA

Plasmid

#48884

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Publication

Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmyc-TNRC6B

Plasmid

#10978

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

TNRC6B (TNRC6B Human)

Tags

myc

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSMP-EZH2_3

Plasmid

#36389

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
TPST2-EGFP

Plasmid

#66618

Purpose

Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

Depositor

David Stephens

Publication

Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

Insert

TPST2 (TPST2 Human)

Expression

Mammalian

Mutation

Insert contains T370A relative to NM_001008566.1

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Ki67-mNeonGreen-IRESpuro2

Plasmid

#183737

Purpose

Expresses Ki67-mNeonGreen

Depositor

Daniel Gerlich

Publication

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His6TEV-UbG76C

Plasmid

#75299

Purpose

Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

Depositor

Cynthia Wolberger

Publication

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

human Ubiquitin mutant G76 to C

Tags

His

Expression

Bacterial

Mutation

changed Glycine 76 to Cystine

Promoter

T7

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pATetO 6XHis T5 Exonuclease

Plasmid

#206209

Purpose

Inducible expression of T5 exonuclease

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

T5 exonuclease

Expression

Bacterial

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mito-gTEMP_pcDNA3

Plasmid

#109117

Purpose

Mitochondria targetting gTEMP

Depositor

Takeharu Nagai

Publication

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

mito-gTEMP

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-Galectin-8 FL

Plasmid

#68805

Purpose

Express Galectin-8 FL protein in Bacterial cells

Depositor

Hyun Kyu Song

Publication

Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

Insert

Galectin-8 (LGALS8 Human)

Tags

N-terminal 6-histidine-tag

Expression

Bacterial

Mutation

No mutation

Promoter

T7

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNF02-mChartreuse

Plasmid

#219397

Purpose

Constitutive expression of mChartreuse in E. coli

Depositor

Christian Lesterlin

Publication

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mChartreuse

Expression

Bacterial

Promoter

proDp

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-dyskerin

Plasmid

#53607

Purpose

Retroviral expression of human dyskerin

Depositor

Steven Artandi

Publication

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

dyskerin (DKC1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR iSH-YFP-PIF

Plasmid

#50841

Purpose

Optogenetic PI3K activation system; cytoplasmic component

Depositor

Wendell Lim

Publication

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Inserts

p85 iSH2
PIF6 1-100

Use

Lentiviral and Synthetic Biology

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAd5-B1

Plasmid

#122551

Purpose

Block 1 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Publication

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Promoter

None

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/p-bacteria

Plasmid

#64325

Purpose

Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Publication

Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

Insert

3xFLAG-dCas9

Use

CRISPR; Tetracycline-inducible

Tags

3xFLAG

Expression

Bacterial

Mutation

D10A, H840A

Promoter

pLtetO-1

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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