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Plasmid#134938PurposeLow phosphate inducible circular permutation of GFPDepositorInsertGFPcp6
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL25
Plasmid#70253PurposeShuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strainsDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL1383a
Plasmid#70692PurposeRSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPMW-attB
Plasmid#61814PurposepUASp plasmid with attB for Drosophila transgenesisDepositorTypeEmpty backboneUseTags6xMycExpressionInsectMutationPromoterUASpAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQF-mNGsacB
Plasmid#203673PurposepQF with mNeonGreen and sacB under control of PQ5DepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQ5Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-SaDAH
Plasmid#140128PurposeExpresses SaDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Sinomenium acutum
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQFT-lacZ
Plasmid#208896PurposepQFT with lacZ under control of PQJDepositorInsertlacZ
UseTagsExpressionBacterialMutationContains two silent mutations and a missense muta…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSgk1_v2 Flag
Plasmid#86040Purposefull length human Sgk1_i2 with Flag tagDepositorInsertAlternate transcript v2 of human Sgk1 (SGK1 Human)
UseTagsFLAGExpressionMammalianMutationv2PromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
NCVB-ssGrl1-ESCargo*
Plasmid#140167PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophilaDepositorInsertssGrl1-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pHCKan-yibDp-GST
Plasmid#134592PurposeLow Phosphate inducible GSTDepositorInsertGlutathione S-transferase
UseTagsnoneExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBUN279
Plasmid#60674Purposeintegration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB siteDepositorInsertald
UseE.coli-mycobacterium integration-proficient vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-mCherry
Plasmid#127058PurposeLow phosphate inducible mCherry with N-terminal linker and His tagDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSgk1_v3 Flag
Plasmid#86039Purposefull length human Sgk1_i3 with Flag tagDepositorInsertAlternate transcript v3 of human Sgk1 (SGK1 Human)
UseTagsFLAGExpressionMammalianMutationv3; S313GPromoterCMVAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Cav (1-61)
Plasmid#22446DepositorInsertCaveolin1 (1-61)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2010AvailabilityAcademic Institutions and Nonprofits only