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pMLS715

Plasmid

#188887

Purpose

Multisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos Arm

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S10

Plasmid

#128594

Purpose

Expresses tagless S10 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S10 (rpsJ E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3

Plasmid

#128591

Purpose

Expresses tagless S3 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S3 E.coli ribosomal protein (rpsC E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2373

Plasmid

#160079

Purpose

Z. mobilis CRISPRi, promoter F, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2371

Plasmid

#160078

Purpose

Z. mobilis CRISPRi, promoter E, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2369

Plasmid

#160077

Purpose

Z. mobilis CRISPRi, promoter D, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2093

Plasmid

#160074

Purpose

Z. mobilis CRISPRi, promoter B, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylI

Plasmid

#48200

Purpose

Expresses CylI (type III PKS) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylI

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET-29b-CylB

Plasmid

#48199

Purpose

Expresses CylB (acyl carrier protein) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylB

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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Membrane protein gRNA library

Pooled Library

#113345

Purpose

Activation library targeting the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform.

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Species

Homo sapiens

Use

Lentiviral

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
Yeast Inducible CRISPRi Library

Pooled Library

#161829

Purpose

Inducible genome-wide CRISPRi library targeting S. cerevisiae genes with 6-12 guide RNAs per gene.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Expression

Yeast

Species

Saccharomyces cerevisiae

Use

CRISPR

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only
nls-EGFP

Plasmid

#67652

Purpose

Mammalian expression vector driving nls-EGFP.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

nls-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV_JAK3_IRES_BFP

Plasmid

#206291

Purpose

Expresses the wild type form of human JAK3.

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (JAK3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-rtTA

Plasmid

#139480

Purpose

Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

rtTA, Tet-ON reverse transactivator

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-cilia-APEX

Plasmid

#73186

Purpose

Expresses cilia-APEX in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

cilia-APEX (Nphp3 Mouse, Glycine max)

Tags

internal GFP tag

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pB80-2xFKBP-L-mCherry-CAAX

Plasmid

#193713

Purpose

Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

2xFKBP-L-mCherry-CAAX

Tags

2xFKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7

Plasmid

#170141

Purpose

Expresses CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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