We narrowed to 16,705 results for: Plat;

1. pcDNA3.1-CMV-PdCO-EGFP

   Plasmid

   #198512

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85844

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   hSyn1

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

   Plasmid

   #183078

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
   

   Use

   AAV; Homologous recombination donor template

   Tags

   Expression

   Mutation

   V600E (T>A), S607S (TCC>AGT)

   Promoter

   None for primary insert. PGK for puromycin resist…

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

   Plasmid

   #60231

   Purpose

   Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   EGFP
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA-P2A and EGFP-KASH

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hSyn

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. xPERT-hA2q

   Plasmid

   #231063

   Purpose

   Circularly permuted CasRx platform fused to human deaminase domain for A to I editing

   Depositor

   Meng How Tan

   Article

   Wang et al Nat Biotechnol. 2024 Oct 9. doi: 10.1038

   Inserts

   dCas13d
   Human deaminase ADAR2dd (ADARB1 Ruminococcus flavefaciens XPD3002)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H460D, E488Q, only the deaminase domain (aa 276-7…

   Promoter

   CMV

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_hSyn-PdCO-EGFP-WPRE

   Plasmid

   #198513

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

   Plasmid

   #198511

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60226

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hSyn-PdCO-mScarlet-WPRE

   Plasmid

   #198510

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

    Plasmid

    #198515

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

    Plasmid

    #198508

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-SIO-PdCO-mScarlet-WPRE

    Plasmid

    #198509

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

    Plasmid

    #198514

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid

    #202199

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid

    #202198

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid

    #183078

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    

    Use

    AAV; Homologous recombination donor template

    Tags

    Expression

    Mutation

    V600E (T>A), S607S (TCC>AGT)

    Promoter

    None for primary insert. PGK for puromycin resist…

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. UQ6106

    Bacterial Strain

    #37176

    Depositor

    Gary Roberts

    Bacterial Resistance

    Ampicillin

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pUC19-link-sfGFP-FLAG:Kan

    Plasmid

    #211833

    Purpose

    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    sfGFP-FLAG-Kan
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-link-mVenus-FLAG:Kan

    Plasmid

    #211830

    Purpose

    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mVenus-FLAG-Kan
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge