We narrowed to 10,582 results for: plasmids 101

1. INT_GFPsp

   Plasmid

   #212144

   Purpose

   Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

   Depositor

   Kang Zhou

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23119

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. INT_GFPsp_CamRcargo

   Plasmid

   #212143

   Purpose

   Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

   Depositor

   Kang Zhou

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23119

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDAX-554 N-Tag GFP[1] L10 Stat3 EBPF2

   Plasmid

   #130101

   Purpose

   PPI (BiFC/TriFC) assay vector

   Depositor

   Gary Lewin

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRW402-P1int-BXB1

   Plasmid

   #235979

   Purpose

   Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.

   Depositor

   Chang Liu

   Article

   Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCM167.2-Venus-VF-hADAM10(aa1-213)

   Plasmid

   #189554

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 1-213

   Promoter

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMBA324

   Plasmid

   #214741

   Purpose

   Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSV015

   Plasmid

   #214738

   Purpose

   Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. INT_Cargo_PgyrA_katG_dadA6

   Plasmid

   #212142

   Purpose

   Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.

   Depositor

   Kang Zhou

   Insert

   catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PgyrA

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-MK1

   Plasmid

   #29638

   Depositor

   Bruce Tempel

   Insert

   Mk1 (Kcna1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid

    #189560

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-654

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCM167-Venus-VF-hADAM10(aa1-459)

    Plasmid

    #189559

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-459

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCM167.2-Venus-VF-hADAM10(aa455-550)

    Plasmid

    #189556

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 455-550

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCM167-Venus-VF-hADAM10(aa220-459)

    Plasmid

    #189555

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-459

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCM167.2-Venus-VF-hADAM10(aa551-654)

    Plasmid

    #189557

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa551-654

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid

    #189562

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCM167-Venus-VF-hADAM10(aa455-654)

    Plasmid

    #189561

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 455-654

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCM167.2-Venus-VF-hADAM10(aa1-654)

    Plasmid

    #189558

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain through cysteine rich domain--amino acids 1-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa1-654

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CBX7

    Plasmid

    #25241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX7_04 (CBX7 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

    Plasmid

    #229223

    Purpose

    For expression of T. elongatus KaiB protein mutant

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    1-94 (truncated), Y8A, Y94A

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZP17 Krox20

    Plasmid

    #16924

    Depositor

    Randall Moon

    Insert

    Krox20 (egr2b Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only