-
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
-
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
UseTagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-DAAT-V33G/T242G
Plasmid#159278PurposeAminotransferase variant with near-native catalytic efficiency toward D-tryptophanDepositorInsertEngineered d-alanine aminotransferase
UseTags6x His tagExpressionBacterialMutationValine 33 and threonine 242 have been mutated to …PromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 V142A-mycDDK
Plasmid#137768PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 V142A
UseTagsmycDDKExpressionMammalianMutationV142APromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 G120
Plasmid#123077PurposeGateway entry clone encoding human MAP1LC3B2 G120DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acids 121-125. Stop codon after G12…PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 G120A
Plasmid#123076PurposeGateway entry clone encoding human MAP1LC3B2 G120ADepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 120 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopTagsExpressionMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPTI-21
Plasmid#195058Purposesimplyfied amino acid sequence by alanine scanningDepositorInsertbovine pancreatic trypsin inhibitor
UseTagsHis tag and trpΔLE leader sequencesExpressionBacterialMutation21 amino acids are Alanine out of 58 amino acidsPromoterT7 promotorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0765
Plasmid#177031PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInserttryptophan decarboxylase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 L140A-mycDDK
Plasmid#137767PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 L140A
UseTagsmycDDKExpressionMammalianMutationL140APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 R12H-mycDDK
Plasmid#137770PurposeCALCOCO1 R12H mutantDepositorInsertCALCOCO1 R12H
UseTagsmycDDKExpressionMammalianMutationR12HPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 no-stop
Plasmid#123207PurposeGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPa-c6MYCn
Plasmid#69242PurposeTet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP-c6MYCn
Plasmid#69243PurposeTet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS3+4)
Plasmid#69246PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#133786PurposeCre-dependent tdTomato fluorescent reporter for cell labelingDepositorInsertpAAV-EF1a-DIO-tdTomato-WPRE
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C WT
Plasmid#123078PurposeGateway entry clone encoding human MAP1LC3C wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only