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TAL3272

Plasmid

#42735

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-gper-left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3393

Plasmid

#42856

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vasa-right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3392

Plasmid

#42855

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vasa-left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pHInt-AppA-KanR-loxP

Plasmid

#232910

Purpose

Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Publication

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

AppA (appA )

Use

Synthetic Biology

Promoter

Pj5

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII250

Plasmid

#205025

Purpose

Expressing engineered M.EcoGII with higher thermostability

Depositor

Paola Laurino

Publication

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 250

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-NES-mTagBFP2

Plasmid

#216129

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

Depositor

Stefan Kubicek

Publication

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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ykgMO-IGS-LuxCDABE

Plasmid

#207232

Purpose

Calprotectin sensing promoter with luciferase reporter

Depositor

Arthur Prindle

Publication

Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

ykgMO-IGS

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox2-3xflag

Plasmid

#206376

Purpose

Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Publication

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3TOPO-T7-hCas9

Plasmid

#161876

Purpose

In vitro transcription of SpCas9

Depositor

Stephane Pelletier

Publication

Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

Insert

human codon optimized SpCas9

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Publication

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2215 - Intron GG3 - 900 bp

Plasmid

#159882

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Publication

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI036

Plasmid

#105350

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

LexA operator & 35S min promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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FRP1671

Plasmid

#64538

Purpose

Inducible MET15 gene

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

MET15 (MET17 Budding Yeast)

Expression

Yeast

Promoter

(lexA-box)_4-P_CYC1_minimal

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBW412hrpL-cIgfp

Plasmid

#61438

Purpose

pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs32-CI-LVA-t-plam-30gfp-t

Tags

LVA

Expression

Bacterial

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC40

Plasmid

#62334

Purpose

sgRNA + 2x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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Gal4pBS34-Pleum

Plasmid

#60334

Purpose

Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTXG-hXRCC1

Plasmid

#52283

Purpose

Bacterial expression of human XRCC1 C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDUET-ctCPR-A13AMO

Plasmid

#20114

Depositor

Jay Keasling

Publication

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pETMutS-E41A

Plasmid

#13048

Depositor

Peggy Hsieh

Publication

Schofield et al J Biol Chem. 2001 Dec 7. 276(49):45505-8

Insert

MutS

Expression

Bacterial

Mutation

mutation Glu41 to Ala full-length

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only