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We narrowed to 14,352 results for: Cas9

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Showing: 5621 - 5640 of 14352 results

  1. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117168-plasmid-map-sequence-id-238529

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  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  7. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    U6 and synthetic Probasin ARRx2
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68357-plasmid-map-sequence-id-122833

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  9. ABE9-SpRY

    Plasmid
    #242976
    Purpose
    A-to-G base editor fused to PAM-flexible SpRY-Cas9 nickase.
    Depositor
    Marc Freichel
    Publication
    Ong et al Sci Rep. 2026 Feb 20;16(1):7463. doi: 10
    Insert
    pCMV-TadA-8e (N108Q/L145T)-nSpRYCas9
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    6*His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242976-plasmid-map-sequence-id-486100

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  15. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Publication
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Expression
    Yeast
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196040-plasmid-map-sequence-id-386150

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  16. pEvolvR-mCherry-eNme2.C.NR-nCas9(D16A)-PolI5MΔ

    Plasmid
    #249077
    Purpose
    Expresses nucleus-localized eNme2.C.NR-nCas9 (D16A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    eNme2.C.NR-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249077-plasmid-map-sequence-id-496316

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  17. pLV-hU6-ZNF865-hUbC-dCas9-KRAB-eGFP

    Plasmid
    #241308
    Purpose
    3rd gen lenti vector encoding dCas9 (s. Pyogenes) fused with the KRAB and a 2A eGFP fluorescence marker. Includes guide for suppression of the ZNF865 gene
    Depositor
    Robby Bowles
    Insert
    ZNF865 sgRNA (ZNF865 Human)
    Use
    Lentiviral
    Available since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    241308-plasmid-map-sequence-id-483070

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  20. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

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Showing: 5621 - 5640 of 14352 results