We narrowed to 14,099 results for: cas9

1. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-PtH2B

   Plasmid

   #133390

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   No promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCE35

   Plasmid

   #174409

   Purpose

   C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CP1041-ABEmax

   Plasmid

   #119808

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PL552

   Plasmid

   #68407

   Purpose

   Donor empty vector with floxed puromycin resistance gene expression cassette

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   mouse PGK promoter

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-CPspaH2B

   Plasmid

   #133385

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYN2_1

   Plasmid

   #184757

   Purpose

   Plasmid for genome editing by CRISPR/Cas9

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   Cas9
   sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
   

   Use

   Synthetic Biology

   Tags

   2xNLS

   Expression

   Bacterial and Yeast

   Promoter

   PGK1 and tRNA-Phe-HDV Ribozyme

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFSW FLEx mCherry-KASH

   Plasmid

   #139652

   Purpose

   Conditional expression of mCherry-KASH

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   mCherry-KASH
   

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBHM2404

    Plasmid

    #173441

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-NAT1
    

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBHM2406

    Plasmid

    #173442

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-HYGB
    

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgTrack-mCherry

    Plasmid

    #114013

    Purpose

    Empty sgRNA expression vector with co-expression of mCherry reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTLR repair vector

    Plasmid

    #64322

    Purpose

    Vector for repair of the traffic light reporter (TLR), providing the venus coding region

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    Venus
    

    Use

    Artificial targeting vector

    Mutation

    see publication for details

    Promoter

    none

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-puro U6 sgRNA CAG

    Plasmid

    #50927

    Purpose

    U6 driven sgRNA negative control

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    negative control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTA-HUH

    Plasmid

    #221152

    Purpose

    HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Insert

    HisG-URA3-HisG
    

    Expression

    Bacterial and Yeast

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPEY-GAL-Nat

    Plasmid

    #232097

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ167

    Plasmid

    #69309

    Purpose

    Gateway entry vector with Cas9p

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    Cas9p (Plant codon-optimized; high GC content at 5 prime region)
    

    Use

    CRISPR

    Mutation

    NLS fusion

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCE38

    Plasmid

    #174432

    Purpose

    C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgTrack-GFP

    Plasmid

    #114012

    Purpose

    Empty sgRNA expression vector with co-expression of TurboGFP reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge