We narrowed to 11,028 results for: HA

1. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Cre-T2A-mRuby2

   Plasmid

   #102989

   Purpose

   Expresses Cre and mRuby2

   Depositor

   Karin Scharffetter-Kochanek

   Article

   Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

   Inserts

   Cre (site-specific recombinase)
   mRuby2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCGN-ATF6 (1-373)

   Plasmid

   #27173

   Purpose

   For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   ATF6 (ATF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains only aa 1-373 (please see depositor comm…

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gαi1-LgB91

   Plasmid

   #134342

   Purpose

   Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi1-LgB91 (GNAI1 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-ABEmax-NRTH

   Plasmid

   #136922

   Purpose

   Expresses ABEmax-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Frankenbody-FLAG-SNAP

   Plasmid

   #175295

   Purpose

   Track mature FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with SNAP-tag
   

   Tags

   SNAP-tag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCMV sMTase (eGFP)

   Plasmid

   #89930

   Purpose

   Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC-IRES-MN
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   deactivated Cas9

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-ABEmax-NRCH

   Plasmid

   #136923

   Purpose

   Expresses ABEmax-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRCH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CHRNA7-mGFP

   Plasmid

   #62629

   Purpose

   mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   hAlpha7 (CHRNA7 Human)
   

   Tags

   mEGFP inserted in M3-M4 loop

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. dxCas(3.7)-VPR

    Plasmid

    #108383

    Purpose

    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.7)-VPR
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-H-Ras_V12

    Plasmid

    #39504

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-CMV-TetO2_HA-BirA-ER

    Plasmid

    #113897

    Purpose

    Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-UBC-mCD24-T2A-GFP

    Plasmid

    #205448

    Purpose

    lentiviral plasmid for expression of mouse CD24

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD24 (Cd24a Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-CMV-TetO2_HA-BirA

    Plasmid

    #113896

    Purpose

    Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1.Gα13-LgB106

    Plasmid

    #134364

    Purpose

    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα13-LgB106 (GNA13 Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-Sec24D

    Plasmid

    #32677

    Depositor

    Henry Lester

    Article

    Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

    Insert

    Sec24D (SEC24D Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. hGATA-1 WT

    Plasmid

    #118352

    Purpose

    This plasmid expresses human GATA-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-ABE7.9

    Plasmid

    #102918

    Purpose

    expresses ABE7.9 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.9
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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