166,461 results

1. V5-TurboID-NES_pCDNA3

   Plasmid

   #107169

   Purpose

   expresses V5-tagged TurboID in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag-β-Lactamase-SpyCatcher

   Plasmid

   #52656

   Purpose

   Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

   Depositor

   Mark Howarth

   Article

   Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

   Insert

   TEM-1 β-Lactamase
   

   Use

   Tags

   SpyCatcher and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-G-Flamp1

   Plasmid

   #188567

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE puro human IRS-1

   Plasmid

   #11025

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Strep-KIFC1*-mCh

   Plasmid

   #120169

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. XLone-Puro Cas13d-eGFP U6 BbsI

   Plasmid

   #155184

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Unspecified; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Halo-TOMM20-N-10

    Plasmid

    #123284

    Purpose

    Localization: Outer Mitochondrial Membrane

    Depositor

    Kevin McGowan

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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