We narrowed to 7,496 results for: addgene/1000

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Lenti-sgKeap1#2/Cre

Plasmid

#173640

Purpose

Expresses a Keap1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Keap1 (Keap1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-proα2(I)-G610C

Plasmid

#119841

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mApple

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX RASGAP(C)-SH2

Plasmid

#46431

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(C) (RASA1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 339-452

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX RASGAP(NC)-SH2

Plasmid

#46433

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(NC) (RASA1 Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 171-448

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

Plasmid

#110761

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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MEK1C218

Plasmid

#164639

Purpose

Bacterial expression plasmid for His6-MEK1C218

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S218C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS-Neu HA SIINFEKL

Plasmid

#125832

Purpose

Retroviral expression of rat Neu cDNA

Depositor

Yi Li

Article

Du et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

Insert

Neu (Erbb2 Rat)

Use

Retroviral

Tags

2x HA and SIINFEKL

Expression

Mammalian

Mutation

Val to Glu point mutation of codon 664 and trunca…

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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hu-TLR4 299 snp-flag pDEST40

Plasmid

#42647

Purpose

destination vector for expression of human TLR4 299 SNP in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 299 SNP (TLR4 Human)

Tags

Flag

Expression

Mammalian

Mutation

D299G

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1162

Plasmid

#29077

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple94 (GPR88 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Aug. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
Stx3 triple mutant (F31A D33A E34A)

Plasmid

#86945

Purpose

Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

Depositor

Thomas Weimbs

Article

Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

Insert

STX3 triple mutant (F31A D33A E34A) (STX3 Human)

Tags

myc-His6

Expression

Mammalian

Mutation

triple mutant (F31A D33A E34A)

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT3-T2A-Cre

Plasmid

#189008

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF and Cre recombinase, separated by T2A motifs

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaSMA_ALFA_231232

Plasmid

#250441

Purpose

Expresses tagged alpha smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Smooth Muscle Actin (ACTA2 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor MAFA-P2A-tdTomato

Plasmid

#246654

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human MAFA locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by MAFA homology regions (MAFA Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-IRES2-CRY2-STIM1(238-448)

Plasmid

#248295

Purpose

An expression construct encoding a CRY2-fused STIM1 fragment (a.a.238-448) linked to EGFP through an IRES2 element.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP and CRY2-fused STIM1 fragment (a.a.238-448) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-237,449-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnas

Plasmid

#245994

Purpose

Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnas (Gnas Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreen

Plasmid

#244172

Purpose

Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (75S) (KDR Synthetic, Human)

Use

Synthetic Biology

Tags

VEGFR2 signal peptide - 3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUltra BCR-ABL WT

Plasmid

#210432

Purpose

Lentiviral expression of BCR-ABL WT

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

BCR-ABL (ABL1, BCR Human)

Use

Lentiviral

Tags

EGFP-P2A

Promoter

UbC

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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