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Danio rerio cryabb

Plasmid

#47665

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha B-crystallin (cryabb Zebrafish)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFa.1-DN-GFP

Plasmid

#37110

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Mutation

deleted amino acids 1326-1894

Promoter

UAS-E1b

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFa.1-DN-GFP

Plasmid

#37110

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Mutation

deleted amino acids 1326-1894

Promoter

UAS-E1b

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

Plasmid

#217689

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE1 (EMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-584

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

Plasmid

#217689

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE1 (EMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-584

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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GAE teo-GFP

Plasmid

#83958

Purpose

Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

TetO-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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GAE teo-GFP

Plasmid

#83958

Purpose

Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

TetO-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCK671.306

Plasmid

#192641

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCK671.306

Plasmid

#192641

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS2B

Plasmid

#145344

Purpose

Cell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS4B

Plasmid

#145347

Purpose

Cell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS2B

Plasmid

#145344

Purpose

Cell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS4B

Plasmid

#145347

Purpose

Cell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK-E3mut

Plasmid

#29691

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK

Tags

Flag and GFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site mutated

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 GFP-GSK3-MAPK-E3mut

Plasmid

#29691

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK

Tags

Flag and GFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site mutated

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA2_ZIKV PrM

Plasmid

#145360

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

March 23, 2024

Availability

Academic Institutions and Nonprofits only

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