We narrowed to 9,299 results for: yeast expression

1. pCSJ982

   Plasmid

   #176622

   Purpose

   Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

   Insert

   Ub-MPGLW-Fbp1
   

   Use

   Tags

   Ubiquitin N-terminal fusion and triple flag tag C…

   Expression

   Yeast

   Mutation

   Changed first 4 N-terminal amino acid residues of…

   Promoter

   TDH3tc3

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS1576

   Plasmid

   #239524

   Purpose

   Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

   Depositor

   John Dueber

   Insert

   ScVba2p-mTurquoise2-2xCrk_SH3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPAeBlueHPV16LR

   Plasmid

   #185893

   Purpose

   2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PALD6>AeBlue>TPGK1- PSe.GAL2> HPV16-L1_C-6*H > TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYC6Lys-TRP1URA3

   Plasmid

   #11010

   Depositor

   Francisco Malagon

   Insert

   Pgal1TRP1URA3
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 fused to ORF-URA without stop codon

   Promoter

   Available Since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYC6H-TRP1URA3

   Plasmid

   #11009

   Depositor

   Francisco Malagon

   Insert

   Pgal1TRP1URA3
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 fused to ORF-URA without stop codon

   Promoter

   Available Since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKS1336

   Plasmid

   #239510

   Purpose

   Express Hut1p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

   Depositor

   John Dueber

   Insert

   ScHut1p-mTurquoise2-2xCrk_SH3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKS1466

   Plasmid

   #239516

   Purpose

   Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR

   Depositor

   John Dueber

   Insert

   ScErg20p(F96W_N127W)-ePTS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKS1137

   Plasmid

   #239498

   Purpose

   Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur

   Depositor

   John Dueber

   Insert

   sfCherry3C(1-10)-ePTS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS1135

   Plasmid

   #239497

   Purpose

   Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2

   Depositor

   John Dueber

   Insert

   Ost1ss-sfCherry3C(11)-Pex3_TMD(+/-6AA)-2xCrk_SH3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKS1169

    Plasmid

    #239503

    Purpose

    Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    sfCherry3C(1-10)-dPTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS1025

    Plasmid

    #239496

    Purpose

    Express inactive peroxisomal RsAS as negative control

    Depositor

    John Dueber

    Insert

    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H360E

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS416 GAL1pro-YFP

    Plasmid

    #224875

    Purpose

    Low copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)

    Depositor

    Paul Herman

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RPL3-GFP (96)

    Plasmid

    #24040

    Depositor

    Michael Rout

    Insert

    RPL3 (RPL3 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

14. LHP556 - UBC4

    Plasmid

    #32440

    Depositor

    Linda Hicke

    Insert

    UBC4 (UBC4 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UBC4

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. LHP1472 - EDE1

    Plasmid

    #32238

    Depositor

    Linda Hicke

    Insert

    EDE1 (EDE1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P-lac

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

16. PHO4NLS-GFP-PRA (109)

    Plasmid

    #24044

    Depositor

    Michael Rout

    Insert

    PHO4
    

    Use

    Tags

    EGFP and PrA

    Expression

    Yeast

    Mutation

    Only bp 421-588 of PHO4 (NLS)

    Promoter

    Available Since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

17. NAB2NLS-GFP-PRA (107)

    Plasmid

    #24043

    Depositor

    Michael Rout

    Insert

    NAB2
    

    Use

    Tags

    EGFP and PrA

    Expression

    Yeast

    Mutation

    Only bp 601-750 of NAB2 (NLS)

    Promoter

    Available Since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pBD_PYR1_156-F1

    Plasmid

    #241286

    Purpose

    Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;

    Depositor

    Sean Cutler

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS424 Prp8 S1584F

    Plasmid

    #234122

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S1584F

    Promoter

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS424 Prp8 S1584Y

    Plasmid

    #234121

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S1584Y

    Promoter

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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