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Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
UseTagsExpressionBacterial and YeastMutationPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMonAID_dCas9-PmCDA_Hyg_ALS
Plasmid#91692PurposeMonocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A and H840A for dead Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
Plasmid#192160PurposeDual AAV vector AAV-dCas9DepositorInsertDead Cas9
UseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Agarabi CHO Light Chain in pJ201
Plasmid#111566PurposeCloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell lineDepositorInsertMurine light chain
UseSynthetic Biology; Cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ACBEmax
Plasmid#139106PurposeMammalian ACBEmax expression vector (pSI-632)DepositorInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVC299 VEGF Site#2
Plasmid#47506Purposehuman gRNA expression vector targeting VEGFDepositorInsertgRNA-VEGF site 2 (VEGFA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-d
Plasmid#136299Purposeexpresses ABE8.17-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
BMR1_01g02031-bio-His
Plasmid#108116Purpose[Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tagDepositorInsertBMR1_01g02031
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
A*03:02:01
Plasmid#203294PurposeOverexpress HLA alleleDepositorInsertA*03:02:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B3
Plasmid#103004Purposenon-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B3 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
B*58:02:01
Plasmid#203262PurposeOverexpress HLA alleleDepositorInsertB*58:02:01 (HLA-B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*66:01:01
Plasmid#203291PurposeOverexpress HLA alleleDepositorInsertA*66:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B2
Plasmid#103003Purposenon-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE3∆UGI
Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149423)
Plasmid#80255Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-IFITM3g1 (BB15)
Plasmid#139460PurposeLentiviral vector with gRNA targeting IFITM3; includes puromycin selectable markerDepositorInsertIFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SARS_COV2_nsp9_HA_BASU
Plasmid#153986PurposeExpress NSP9 gene along with HA and BASUDepositorInsertnsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS_COV2_nsp5_HA_BASU
Plasmid#153987PurposeExpress NSP5 gene along with HA and BASUDepositorInsertnsp5 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Csk-SH2
Plasmid#46420DepositorInsertCsk (CSK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 74-177PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-(STChRger2-TS-EYFP)
Plasmid#129394PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.DepositorInsertsoma targeted ChRger2
UseAAV and Cre/LoxTagsKv2.1-TS-EYFPExpressionMammalianMutationPromoterCAGAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11055
Plasmid#68252PurposeLevel 1 Golden Gate Cassette: Plant kanamycin resistance cassetteDepositorInsertPromoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3s-ABE7.10
Plasmid#113128Purposeexpressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimizedDepositorInsertABE 7.10
UseTagsHA epitope and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
SARS_COV2_N_HA_BASU
Plasmid#153989PurposeExpress N gene along with HA and BASUDepositorInsertSARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-Y132D
Plasmid#113425PurposeExpresses hA3A-BE3-Y132D in mammalian cellsDepositorInserthA3A-BE3-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_Y132DPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29 (LM2708)
Plasmid#208965PurposeUnfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3-PGK-Puro
Plasmid#110845PurposeLentiviral vector for dox-inducible expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS_COV2_nsp2_HA_BASU
Plasmid#153988PurposeExpress NSP2 gene along with HA and BASUDepositorInsertnsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
JpExpress404 PTEN
Plasmid#49420PurposeBacterial Expression VectorDepositorInsertPTEN (PTEN )
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SEPHS2 gRNA (BRDN0001148921)
Plasmid#77661Purpose3rd generation lentiviral gRNA plasmid targeting human SEPHS2DepositorInsertSEPHS2 (SEPHS2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only