We narrowed to 19,002 results for: Tec

1. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRT498-OtUBD

   Plasmid

   #190089

   Purpose

   Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.4_1G11F2-3 heavy chain

   Plasmid

   #198253

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.pU1a-SpCas9

   Plasmid

   #121507

   Purpose

   Expresses codon-optimized SpCas9 in mammalian cells.

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB451

   Plasmid

   #74833

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM1202

   Plasmid

   #69615

   Purpose

   inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aac
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Expression

   Bacterial

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b-TEV-C9R

   Plasmid

   #118754

   Purpose

   TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

   Depositor

   Yutaka Kuroda

   Article

   Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

   Insert

   Tobacco Etch Virus protease (NEWENTRY Synthetic)
   

   Tags

   C9R and His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CP1041-ABE8e

   Plasmid

   #138493

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1041)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTMVi-M

    Plasmid

    #118757

    Purpose

    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.

    Depositor

    Jose-Antonio Daros

    Article

    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

    Insert

    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    

    Use

    Intermediate to build tobacco mosaic virus recomb…

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-RAB-EKARev

    Plasmid

    #118474

    Purpose

    ddRFP-based single-color biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB414

    Plasmid

    #74808

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL3402 (pTnsABf_Pse)

    Plasmid

    #200901

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsA-TnsB
    

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. APEX2-GBP

    Plasmid

    #67651

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. 35S-GWY-VP16/pAM

    Plasmid

    #201232

    Purpose

    Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GWY-VP16
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL1749 (pDonor, R*MmeI)

    Plasmid

    #160733

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA
    

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAS-V5-TurboID-NES

    Plasmid

    #116904

    Purpose

    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID
    

    Use

    Drosophila expression

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-Clathrin LC-15

    Plasmid

    #55019

    Purpose

    Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin LC (CLTB Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTMVi-N

    Plasmid

    #118756

    Purpose

    Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.

    Depositor

    Jose-Antonio Daros

    Article

    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

    Insert

    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    

    Use

    Intermediate to build tobacco mosaic virus recomb…

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB514

    Plasmid

    #74856

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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