We narrowed to 6,141 results for: arl

1. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-Coff/Fon-BFP

   Plasmid

   #137131

   Purpose

   Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Con/Fon-BFP

   Plasmid

   #137129

   Purpose

   Intersectional viral expression of BFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-FLPo-Myc

   Plasmid

   #124641

   Purpose

   AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   FLPo
   

   Use

   AAV and Cre/Lox

   Tags

   myc tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE GFP-Ago2 S387A

   Plasmid

   #60654

   Purpose

   GFP-Ago2 S387A mutant lentiviral vector

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   S387A

   Promoter

   EF1a

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. optoFcR

   Plasmid

   #224291

   Purpose

   Expression of an optogenetic Fc Receptor

   Depositor

   Meghan Morrissey

   Article

   Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

   Insert

   Fc receptor gamma (Fcer1g Mouse)
   

   Use

   Lentiviral

   Tags

   mScarlet-Cry2-olig and myristoylation sequence

   Mutation

   aa 45-86 only

   Promoter

   SFFV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLP-PIGA2 (pLP300)

   Plasmid

   #168462

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Promoter

   CMV immediate early

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-rsChRmine-eYFP-WPRE

   Plasmid

   #183531

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   rsChRmine-eYFP
   

   Use

   AAV

   Mutation

   I146M/G174S

   Promoter

   CaMKIIa

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-Coff/Fon-sRGECO

    Plasmid

    #137128

    Purpose

    Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    E217D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

    Plasmid

    #137165

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    3x-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    Removed RSET tag

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nEF-Con/Fon-bREACHes-EYFP

    Plasmid

    #137145

    Purpose

    Intersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    Con/Fon-bREACHes-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX 4T-1 GST-Ago2 S387A

    Plasmid

    #60656

    Purpose

    Expresses GST-Ago2 S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S387A

    Promoter

    tac

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa-hsChRmine-eYFP-WPRE

    Plasmid

    #183530

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    hsChRmine-eYFP
    

    Use

    AAV

    Mutation

    H33R

    Promoter

    CaMKIIa

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-LmoCas6-p300

    Plasmid

    #126486

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6-p300
    

    Use

    CRISPR

    Tags

    Flag and Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-plk1-AKAP

    Plasmid

    #132967

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
    

    Expression

    Mammalian

    Mutation

    C terminus of full-length Plk1 fused to the peric…

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE GFP-Ago2 S387E

    Plasmid

    #60655

    Purpose

    GFP-Ago2 S387E mutant lentiviral vector

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    S387E

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-nEF-Coff/Fon-bREACHes-EYFP

    Plasmid

    #137147

    Purpose

    Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    Coff/Fon-bREACHes-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-CasE-p300-Flag

    Plasmid

    #106275

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE-p300
    

    Use

    CRISPR

    Tags

    E tag, NLS and Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-FRET_PST-C

    Plasmid

    #228570

    Purpose

    Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Pancreastatin

    Depositor

    Geert van den Bogaart

    Article

    Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

    Insert

    Chromogranin-A (CHGA Human)
    

    Tags

    mCitrine and mScarlet-I

    Expression

    Mammalian

    Mutation

    FRET sensor; see explanatory note

    Promoter

    cmv

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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