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Showing: 1121 - 1160 of 1428 results

  1. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65216-plasmid-map-sequence-id-115626

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  2. pSR651

    Plasmid
    #65213
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-1 cap
    Use
    AAV
    Tags
    Expression
    Insect
    Mutation
    Promoter
    p10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65213-plasmid-map-sequence-id-112889

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  3. hSyn-Aion-ts-mScarlet-ER

    Plasmid
    #192577
    Purpose
    AAV-vector for optogenetic long-term silencing of neurons
    Depositor
    Simon Wiegert
    Insert
    Aion
    Use
    AAV
    Tags
    ts-mScarlet-ER
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192577-plasmid-map-sequence-id-379289

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  4. CaMKII-Aion-ts-citrine-ER

    Plasmid
    #192576
    Purpose
    AAV-vector for optogenetic long-term silencing of excitatory neurons
    Depositor
    Simon Wiegert
    Insert
    Aion
    Use
    AAV
    Tags
    ts-Citrine-ER
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192576-plasmid-map-sequence-id-379301

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  7. Ef1a-DIO-somAion-citrine

    Plasmid
    #192573
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somAion
    Use
    AAV
    Tags
    Citrine - soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192573-plasmid-map-sequence-id-379368

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  8. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

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  11. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

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  13. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  14. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Article
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Rep2
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    68837-plasmid-map-sequence-id-121219

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  16. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

    Plasmid
    #107049
    Purpose
    All-in-one vectors expressing both SaCas9 and LacZ sgRNA
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107049-plasmid-map-sequence-id-208585

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  17. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid
    #107050
    Purpose
    All-in-one vectors expressing both SaCas9 and YFP sgRNA
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107050-plasmid-map-sequence-id-208586

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  18. PHP.AX

    Plasmid
    #195218
    Purpose
    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)
    Depositor
    Viviana Gradinaru
    Article
    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103
    Insert
    Synthetic construct isolate AAV-PHP.AX VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p41
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195218-plasmid-map-sequence-id-385556

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  19. pTR-UF11

    Plasmid
    #157970
    Purpose
    AAV vector plasmid containing the Chimeric CMV/Chicken Beta Actin promoter driving 'humanized' GFP
    Depositor
    Sergei Zolotukhin
    Article
    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10
    Has Service
    AAV.A01.T, AAV.A02.T, AAV.A03.T, AAV.A04.T, AAV.A05.T, AAV.A06.T, AAV.A07.T, AAV.A08.T, AAV.A09.T, AAV.A10.T, AAV.A11.T, and AAV.A12.T
    Insert
    'humanized' e.g. codon optimized GFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Available since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    157970-plasmid-map-sequence-id-313812

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  24. pGG-B1

    Plasmid
    #78504
    Purpose
    Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV-B1 capsid
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    78504-plasmid-map-sequence-id-146458

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  27. PE_CO-Mini-C

    Plasmid
    #190337
    Purpose
    Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini-C, PCSK9 +1A insertion pegRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    190337-plasmid-map-sequence-id-374556

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  28. PE_CO-Mini-N

    Plasmid
    #190336
    Purpose
    Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    190336-plasmid-map-sequence-id-374544

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  37. CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

    Plasmid
    #117384
    Purpose
    An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Insert
    GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117384-plasmid-map-sequence-id-225349

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Showing: 1121 - 1160 of 1428 results