We narrowed to 1,257 results for: LIPI

1. pET28a-MSP1D1delltaH4

   Plasmid

   #71713

   Purpose

   Membrane scaffold protein (MSP), helix 4 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCit-APPAPAPPAP-gs

   Plasmid

   #228091

   Purpose

   Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   mCit, APPAPAPPAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABHD16A-mNG

   Plasmid

   #195157

   Purpose

   mammalian expression of ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX303 GFP-PIK3C2B

   Plasmid

   #162001

   Purpose

   Lentiviral expression vector containing PIK3C2B tagged with GFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   siRNA resistance to siRNA#2 with 4 silent mutatio…

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28C-mCherry-YTHDF2

   Plasmid

   #204406

   Purpose

   E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNeonGreen-C1-Nir2-LNS2

   Plasmid

   #220086

   Purpose

   C-terminal PA binding domain of Nir2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM1 (PITPNM1 Human)
   

   Use

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 896-1216

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28C-mCherry-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204403

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-C1-Nir1-LNS2

   Plasmid

   #220084

   Purpose

   high affinity PA biosensor with red fluorophore

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   mCherry-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiMutB3GALT5-blast

   Plasmid

   #208381

   Purpose

   lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nucleotide change in PAM targeting sequence nt 51…

   Promoter

   EF-1 alpha core promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-(-30)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62372

    Purpose

    Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP-9xGGS-Cre-6xHis
    

    Use

    Tags

    (-30)GFP and 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. RFP-PASS

    Plasmid

    #193971

    Purpose

    Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

    Depositor

    Guangwei Du

    Article

    Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

    Insert

    PA biosenor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCit-APPAP-gs

    Plasmid

    #228090

    Purpose

    Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, APPAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNES-EGFP-C1-flex-PABD-Spo20

    Plasmid

    #220081

    Purpose

    Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Use

    Tags

    X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

    Expression

    Mammalian

    Mutation

    amino acids 51-91

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV human IRS-1 CAAX

    Plasmid

    #11238

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

15. SAC1C389SdeltaTMD-FKBP-mCherry

    Plasmid

    #108124

    Purpose

    Recruitable cytosolic inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S; 1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. anxA9-GFP

    Plasmid

    #107198

    Purpose

    Expresses human annexin A9-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

    Insert

    annexin A9 (ANXA9 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Hyper7 in pT3TS-Dest

    Plasmid

    #194296

    Purpose

    In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    Hyper7
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH-nuc-roGFP2-GPXC71S

    Plasmid

    #191760

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    GPX has no peroxidatic Cysteine 71, which is repl…

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX303 GFP-PIK3C2A

    Plasmid

    #162000

    Purpose

    Lentiviral expression vector containing PIK3C2A tagged with GFP

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACE-GIRK4-S

    Plasmid

    #172425

    Purpose

    Expression of full-length, human GIRK4 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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