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We narrowed to 660 results for: TEV protease

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Showing: 561 - 580 of 660 results

  1. Fireworks GFP[PTC+] (AVA2600)

    Plasmid
    #85446
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85446-plasmid-map-sequence-id-171530

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  5. pET-15b-aδNb072

    Plasmid
    #204627
    Purpose
    Expresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.
    Depositor
    James McDonnell
    Article
    Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75
    Insert
    aδNb072
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204627-plasmid-map-sequence-id-416167

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  8. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  10. pEG593

    Plasmid
    #40119
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    R274E, K318E, Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only

  11. pEG628

    Plasmid
    #40120
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa1-244, Exon 2 recoded to be RNAi-resis…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Sept. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  15. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  16. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  17. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  18. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  19. pHIs8-4b-SaDAH::G226D

    Plasmid
    #140130
    Purpose
    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli
    Depositor
    Jing-Ke Weng
    Article
    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:
    Insert
    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    changed Gly226 to Asp226
    Promoter
    T7
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140130-plasmid-map-sequence-id-275079

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Showing: 561 - 580 of 660 results