We narrowed to 2,218 results for: dcas9

1. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_TRE3G-LSD1-dCas9-P2A-mCherry

   Plasmid

   #138462

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   LSD1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCXLE-dCas9VP192-T2A-EGFP-shP53

   Plasmid

   #69535

   Purpose

   Episomal plasmid encoding dCas9VP192 and p53 shRNA

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-GFP-shp53
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-dCas9-ZIM3-KRAB-BFP

   Plasmid

   #196712

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-KRAB(ZIM3)-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-KRAB-dCas9-IRES-BFP

   Plasmid

   #85449

   Purpose

   Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti SYN-dCas9-KRAB-MeCP2

   Plasmid

   #155365

   Purpose

   Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   3x FLAG and 7x His

   Expression

   Mammalian

   Mutation

   Promoter

   SYN

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-TRE3G-dCas9-EGFP-HaloTag

   Plasmid

   #169451

   Purpose

   expression of dCas9-EGFP-HaloTag

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9-EGFP-HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1a

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18T-mini-Tn7T-hph-dCas9

    Plasmid

    #218371

    Purpose

    Expression of dCas9 for CRISPR-interference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtetA

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNTI647 dCas9-Mxi1 TetR KanMX

    Plasmid

    #139474

    Purpose

    Integrates CRISPRi effector and tet-responsive regulator

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Inserts

    dCas9-Mxi1
    Tet Repressor
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1 and pTEF1

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. GCN5 core-dCas9-p300 core

    Plasmid

    #179553

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-dCas9-HA-NLS--GFP-p300

    Plasmid

    #183925

    Purpose

    p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-p300
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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