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pTS2367-Tier1-PhCMV_VP16

Plasmid

#169568

Purpose

Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1011-Tier1-PhCMV-Nluc

Plasmid

#169545

Purpose

Tier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven NanoLuc Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH608-Tier1-PmPGK1-Fluc

Plasmid

#169543

Purpose

Tier-1 vector encoding PmPGK1-driven Fluc (PmPGK1-Fluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX15-Tier1-PhCMV-mTurquoise2

Plasmid

#169512

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein mTurquoise2 (PhCMV-mTurquoise2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven monomeric cyan fluorescent protein mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX12-Tier1-PhCMV-eGFP

Plasmid

#169509

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven enhanced green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX8-Tier1-PhCMV-YPet

Plasmid

#169506

Purpose

Tier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven yellow fluorescent protein Ypet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2403-Tier1-PhCMV-ZeoR

Plasmid

#169495

Purpose

Tier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Sh ble

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2402-Tier1-PhCMV-BlastR

Plasmid

#169494

Purpose

Tier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven blasticidin-S deaminase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2401-Tier1-PhCMV-HygroR

Plasmid

#169493

Purpose

Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven hph

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1021-Tier1-PhCMV-PuroR

Plasmid

#169492

Purpose

Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven puromycin N-acetyl-transferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1018-Tier1-PhCMV-p2A

Plasmid

#169491

Purpose

Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven 2A sequence of porcine teschovirus-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH17-Tier1-PhCMV-myr

Plasmid

#169489

Purpose

Tier-1 vector encoding PhCMV-driven myristoylation (myr) signal peptide expression (PhCMV-myr-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven myrisoylation signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2409-Tier1-PhCMV-FURINsite

Plasmid

#169488

Purpose

Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Furin cleavage site

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2379-Tier1-PhCMV-PEST

Plasmid

#169484

Purpose

Tier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven PEST degradation tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2415-Tier1-PPGK-1xEAAAK

Plasmid

#169482

Purpose

Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven EAAAK-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2412-Tier1-PPGK-2xGS

Plasmid

#169481

Purpose

Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven GS-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2378-Tier1-PhCMV-KDEL

Plasmid

#169479

Purpose

Tier-1 vector encoding PhCMV-driven ER-retention signal KDEL (PhCMV-KDEL-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven ER retention signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH8-Tier1-PhCMV-NLS

Plasmid

#169477

Purpose

Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPPC008.106

Plasmid

#171141

Purpose

Expression of J106 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG-altTAG

Plasmid

#158146

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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