We narrowed to 14,165 results for: nts

1. pIS1-Vim/Actb5UTR-renilla

   Plasmid

   #38238

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Actb promoter (1000 nt upstream) and 5' UTR …

   Promoter

   Endogenous Vim

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pGEX C_K nsp1 CoV2

   Plasmid

   #175516

   Purpose

   Expresses C_K for bioconjugation on nsp1

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   C_K nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-mCherry-p2A-LIC1 Y101A, Y103A

   Plasmid

   #74605

   Purpose

   expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Y101A, Y103A

   Promoter

   SFFV

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX nsp1 R99A CoV2

   Plasmid

   #175515

   Purpose

   For protein expression of R99A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   R99A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX nsp1 K164A/H165A CoV2

   Plasmid

   #175514

   Purpose

   For protein expression of K164A/H165A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   K164A/H165A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX nsp1 R124A/K125A CoV2

   Plasmid

   #175513

   Purpose

   For protein expression of R124A/K125A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   R124A/K125A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX C_K nsp1 K164A/H165A CoV2

   Plasmid

   #175517

   Purpose

   Expresses C_K for bioconjugation on nsp1

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   C_K nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS NL4-3 envFS eGFP 5'CMV Δtat

   Plasmid

   #101347

   Purpose

   tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta Tat

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pECE M2-SNX17 S437A

   Plasmid

   #69812

   Purpose

   Expresses M2-SNX17 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Sorting nexin-17 (SNX17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYO359

    Plasmid

    #235750

    Purpose

    Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Inserts

    VPS34 (VPS34 Budding Yeast)
    VPS15 (VPS15 Budding Yeast)
    

    Use

    Tags

    3xTEV-ZZ

    Expression

    Yeast

    Mutation

    S2A, aa 3-887 and T134A, I851R

    Promoter

    GAL-TDH3

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAR074-control-sfGFP

    Plasmid

    #185032

    Purpose

    J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides of rpmE 5'UTR scrambled, Only fi…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p663-UBC-miniTurbo-V5-ZNF746

    Plasmid

    #207819

    Purpose

    N-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-ZNF746 (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p667-UBC-ZNF746-V5-miniTurbo

    Plasmid

    #207820

    Purpose

    C-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-V5-miniTurbo (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p663-UBC-miniTurbo-V5-ZNF746-C571A

    Plasmid

    #207821

    Purpose

    N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-ZNF746-C571A (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    C571A

    Promoter

    UBC

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-Cas9-ZNF746

    Plasmid

    #207822

    Purpose

    Dual expression of Cas9 and sgRNA targeting ZNF746

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746 sgRNA (ZNF746 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

    Plasmid

    #136912

    Purpose

    DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    pCAG-DmrC-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA4TO-3XFLAG Halo R99A nsp1

    Plasmid

    #175422

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R99A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-Flex-Rev-3xGFP

    Plasmid

    #122500

    Purpose

    Cre-dependent GFP expression.

    Depositor

    Kimberly Ritola

    Insert

    3x GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    each GFP has unique nt sequence

    Promoter

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NK697

    Plasmid

    #120856

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-mTFP-Jumblelw1
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Jumblelw1 allele: T to C at nt 1109 causes L305P …

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NK695

    Plasmid

    #120854

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-Jumblelw1-mTFP
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Jumblelw1 allele: T to C at nt 1109 causes L305P …

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge