Addgene

We narrowed to 13,832 results for: nts

Showing: 561 - 580 of 13832 results

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pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pYO359

Plasmid

#235750

Purpose

Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S2A, aa 3-887 and T134A, I851R

Promoter

GAL-TDH3

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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SNAP-BAF

Plasmid

#227443

Purpose

Mammalian expression of SNAP-BAF

Depositor

David Pellman

Article

Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

Insert

Barrier-to-autointegration factor (BANF1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR074-control-sfGFP

Plasmid

#185032

Purpose

J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Nucleotides of rpmE 5'UTR scrambled, Only fi…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746

Plasmid

#207819

Purpose

N-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-ZNF746-V5-miniTurbo

Plasmid

#207820

Purpose

C-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-V5-miniTurbo (ZNF746 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746-C571A

Plasmid

#207821

Purpose

N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746-C571A (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

C571A

Promoter

UBC

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-ZNF746

Plasmid

#207822

Purpose

Dual expression of Cas9 and sgRNA targeting ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 sgRNA (ZNF746 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EF-1a; U6

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

Plasmid

#136912

Purpose

DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

pCAG-DmrC-NLS-3xFLAG-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken Beta Actin

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R99A nsp1

Plasmid

#175422

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R99A nsp1

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-Rev-3xGFP

Plasmid

#122500

Purpose

Cre dependent GFP expression.

Depositor

Kimberly Ritola

Insert

3x GFP

Use

AAV

Tags

Expression

Mutation

each GFP has unique nt sequence

Promoter

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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NK697

Plasmid

#120856

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Insert

pac-P2A-mTFP-Jumblelw1

Use

Choanoflagellate expression

Tags

Expression

Mutation

Jumblelw1 allele: T to C at nt 1109 causes L305P …

Promoter

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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NK695

Plasmid

#120854

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Insert

pac-P2A-Jumblelw1-mTFP

Use

Choanoflagellate expression

Tags

Expression

Mutation

Jumblelw1 allele: T to C at nt 1109 causes L305P …

Promoter

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB kd K106R

Plasmid

#108493

Purpose

expression of EGFP-AuroraB kinase dead (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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MSP712

Plasmid

#65768

Purpose

Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Mutation

D10A and H840A mutations in Cas9

Promoter

T7 (x2)

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Lacra Bintu, Michael Bassik, Ami Bhatt, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRF2-E79Q

Plasmid

#207828

Purpose

FLAG-tagged NRF2-E79Q driven by CMV promoter

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF2-E79Q (NFE2L2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

E79Q

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pColdI-SBP-eIF4A1

Plasmid

#233621

Purpose

Expression of SBP-tagged eIF4A1 in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

SBP-eIF4A1 (EIF4A1 Human)

Use

Tags

His6-SBP

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_6xH

Plasmid

#186461

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 WT

Plasmid

#69504

Purpose

Expresses AMPK alpha1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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