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Plasmid#232104PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan-ADE2
Plasmid#232105PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-mogat3b in situ probe
Plasmid#232136Purposezebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymeraseDepositorInsertmogat3b
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcRII-mogat3a in situ probe
Plasmid#232135Purposezebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat3a
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-dgat2 in situ probe
Plasmid#232134Purposezebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymeraseDepositorInsertdgat2 (dgat2 Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7
Plasmid#196846Purposebackbone for gRNA cloning of dpspCas13b tagged by 2xPP7DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b
Plasmid#196847Purposebackbone for gRNA cloning of dpspCas13b tagged by 1xMS2DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR 2.1-TOPO Fzd6HA
Plasmid#208363PurposeExtracellular HA-tagged Fzd6 (mouse)DepositorInsertFzd6 (Fzd6 Mouse)
UseCloning vectorTagsHAExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-TRE3G-Nluc
Plasmid#192659PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-NlucDepositorInsertNluc
UseLuciferaseTagsExpressionMammalianMutationPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-miniCMV-Nluc
Plasmid#192657PurposeA plasmid encoding miniCMV sgRNA and miniCMV-promoter-NlucDepositorInsertNluc
UseLuciferaseTagsExpressionMammalianMutationPromoterU6, miniCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-gB-PuroR [M1G]
Plasmid#171803PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-gB-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-gB-BlastR [M1G]
Plasmid#171816PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.DepositorInsertmScarlet-F-gB-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-gB-PuroR [M1G]
Plasmid#171812PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1DepositorInsertmScarlet-F-gB-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-gB-BlastR [M1G]
Plasmid#171807PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1GDepositorInsertmNeonGreen-F-gB-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB-STREP
Plasmid#128499PurposeExpresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.DepositorInsertGRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x) and Strep tag (2x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-Incenp 8KQ
Plasmid#108501Purposeexpression of vsv-INCENP 8KQDepositorInsertINCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationK548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-CenpB 1-166
Plasmid#108474Purposeexpression of vsv-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
UseTagsVSVExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-dCEN-Incenp
Plasmid#108479Purposeexpression of vsv-dCEN-INCENPDepositorInsertdCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box removedPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Sema4b-ISH-2kb
Plasmid#68030PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertSemaphorin-4B (Sema4b Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only