Addgene

We narrowed to 1,648 results for: tyr

Showing: 561 - 580 of 1648 results

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pX330 EGFR-sgRNA

Plasmid

#188633

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 WT

Plasmid

#29536

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
Stat1 alpha Y701F pRc/CMV

Plasmid

#8701

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Y701F

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pTHMT_hnRNPF_FL

Plasmid

#139114

Purpose

express FL hnRNPF with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-Lats1-PY1+2 mutant

Plasmid

#19054

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Large tumor suppressor kinase 1, N mutant (LATS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLD-puro-2A-rtTA-TcVA

Plasmid

#24592

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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MAC_C_LMTK2

Plasmid

#187752

Purpose

MAC-tagged gene expression of human LMTK2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK2 (LMTK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2 Lats1-PY1+2 mutant

Plasmid

#18975

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Large tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
MAC_C_IGF1R

Plasmid

#187770

Purpose

MAC-tagged gene expression of human IGF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

IGF1R (IGF1R Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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ALIX(P801G)-Strep

Plasmid

#180024

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX(1-868, P801G)-Strep (PDCD6IP Human)

Use

Tags

Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

P801G

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP2-SH2

Plasmid

#46493

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP2

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-131

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only
RapR-Shp2-mVenus-flag

Plasmid

#188655

Purpose

Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Shp2

Use

Tags

Flag and mVenus

Expression

Mammalian

Mutation

iFKBP insertion at V406 with deletion of residues…

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-VSV

Plasmid

#139346

Purpose

Expression of kinase-dead TYK2-K930R protein

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSVE Grb2 myc 49L

Plasmid

#26085

Depositor

Bob Weinberg

Article

Egan et al Nature. 1993 May 6. 363(6424):45-51.

Insert

Grb2 P49L (GRB2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

P49L

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFb-DN-GFP

Plasmid

#37111

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

Deleted aa1386-1909 corresponding to phosphatase …

Promoter

10xUAS

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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