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We narrowed to 872 results for: vill

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pCMV-DNAJC5 (L115R)-HaloTag

Plasmid

#205711

Purpose

Mammalian expression of DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2

Plasmid

#185346

Purpose

Membrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

Plasmid

#185347

Purpose

Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RG-Linker-eGFP

Plasmid

#176071

Purpose

EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

RG

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_ZFP64_Delta_TAD

Plasmid

#118698

Purpose

Express ZFP64 without transcription activation domain(TAD)

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64_Delta_TAD (ZFP64 Human)

Use

Lentiviral

Tags

3XFLAG was fused to the N terminal of ZFP64 cDNA

Expression

Mutation

Delete amino acid from 453 to 681

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_LKB1_CR

Plasmid

#108112

Purpose

Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

LKB1 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

change cytosine 333 to alanine (silent mutation)

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgMYB(human)#4.9

Plasmid

#105591

Purpose

lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human MYB DBD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgMYB(human)#5.2

Plasmid

#105592

Purpose

lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human MYB DBD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T-Mst2 SARAH

Plasmid

#84298

Purpose

Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst2 (STK3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L443W

Promoter

tac

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

modified bovine U6-2

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Cre-dependent pan-membrane expression of the genetically encoded voltage indicator ASAP5, can be used for dendritic voltage imaging

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Tags

Expression

Mutation

Promoter

human synapsin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-p21

Plasmid

#171122

Purpose

Doxycycline inducible expression of p21

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

p21 (CDKN1A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDule2 - Mj Acd A9

Plasmid

#197652

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Article

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Use

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A

Plasmid

#145374

Purpose

expresses human Nav1.5 (WT) in mammalian cells

Depositor

Robert Kass, Hugues Abriel

Article

Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-ASAP5-Kv2.1-WPRE

Plasmid

#225707

Purpose

Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5-Kv2.1

Use

AAV

Tags

Expression

Mutation

Promoter

human synapsin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

Plasmid

#225708

Purpose

Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5-Kv2.1

Use

AAV

Tags

Expression

Mutation

Promoter

EF1α

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

Plasmid

#216475

Purpose

lentiviral dual expression of YAP5SA and eGFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
eGFP

Use

Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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