169,450 results
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Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo
Plasmid#221899PurposeExpresses alpha tubulin fused to mStayGoldDepositorInsertmStayGold-aTubulin
ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GMutationS34C mutation in NefAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_JN.1
Plasmid#218678Purposeexpressing SARS-CoV-2 JN.1 spike protein for pseudovirus productionDepositorInsertSpike (JN.1)
ExpressionMammalianMutationL455S on the basis of BA.2.86PromoterCMVAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-AREL1
Plasmid#66710PurposeExpresses AREL1 HECT domain in E. coliDepositorAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
hNTo1-qgRNA-pYJA5
Plasmid#217781PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE
Plasmid#223287PurposeA complete OptoMBP cassette for dissolution of FUS-CHOP condensates.DepositorInsertmyc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
UseSynthetic Biology; Piggybac transposase insertabl…ExpressionMammalianAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2_EF1a_TetOn_IRES_mCherry
Plasmid#204155PurposeLentivirus backbone expressing rtTA linked to mCherryDepositorInsertTet-On 3G
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG5-mCALEBlong (aa 1-566)
Plasmid#89399Purposemammalian expression of mouse CSPG5 long isoformDepositorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458-AAVS1
Plasmid#113194PurposeEncodes gRNA for human AAVS1DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-N1
Plasmid#79987PurposeMonomeric near-infrared fluorescent protein miRFP670 in mammalian plasmidDepositorHas ServiceCloning Grade DNAInsertmiRFP670
ExpressionMammalianPromoterCMVAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGatewayPromoteralpha-Acrystallin promoterAvailable SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT3.0 (AAV8)
Viral Prep#208717-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-GRAB_g5-HT3.0 (#208717). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_g5-HT3.0 plasmid DNA. Synapsin-driven expression of GRAB_g5-HT3.0 sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEx-ER-TurboID (AAV8)
Viral Prep#160857-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-FLEx-ER-TurboID (#160857). In addition to the viral particles, you will also receive purified pAAV-FLEx-ER-TurboID plasmid DNA. CMV-driven, Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEED
Plasmid#219658PurposePermits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)DepositorInsertEmpty SEED Cassette (3xP3-dsRed)
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV Retrograde)
Viral Prep#83896-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (physically separate, not a fusion protein)Available SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
Plasmid#162037PurposeBackground-free mRNA labeling tripartite superfolder GFP systemDepositorInsertGFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
UseLentiviralExpressionMammalianAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only