164,884 results

1. pMGE-T7

   Plasmid

   #153021

   Purpose

   Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

   Depositor

   Vito Valiante

   Article

   Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-jGCaMP7f-WPRE (AAV PHP.eB)

   Viral Prep

   #104488-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Tags

   None

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

3. SPLICS Po-Mt Long P2A

   Plasmid

   #164115

   Purpose

   Detect the long Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2pA2-9xGRE

   Plasmid

   #74736

   Purpose

   GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

   Depositor

   Luisa Dalla Valle

   Article

   Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

   Insert

   EGFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   nine GC-responsive elements (9xGRE) from the rat …

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1-Nucleocapsid

   Plasmid

   #179696

   Purpose

   Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

   Depositor

   Mark Arbing

   Insert

   SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His6-TEV protease site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV5)

   Viral Prep

   #50476-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   CaMKII

   Tags

   mCherry

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEF1a-nSpCas9

   Plasmid

   #171691

   Purpose

   Expresses nSpCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF1a

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone

   Pooled Library

   #1000000075

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of mouse genes

   Depositor

   Feng Zhang

   Available since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSPIH6

    Plasmid

    #190676

    Purpose

    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

    Depositor

    John Vederas

    Article

    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

    Inserts

    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    

    Use

    Tags

    Chitin binding domain, His tag, and N-terminal Hi…

    Expression

    Bacterial

    Mutation

    Added C-terminal his tag and None

    Promoter

    T7

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRN11

    Plasmid

    #84455

    Purpose

    shuttle plasmid for sarA P1-mCherry expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mCherry
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

    Plasmid

    #208361

    Purpose

    Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

    Depositor

    David Bennett

    Article

    Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

    Insert

    mCherry-TANDEM-PSAM4-GlyR
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 MKK7b1(K149A)

    Plasmid

    #14674

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(K149A) (Map2k7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys-149Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

14. GFP-WIPI2d-TEVcs-TSF

    Plasmid

    #171420

    Purpose

    For the purifcation of GFP- WIPI2d protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. eNS2B47NS3 L30SF31S

    Plasmid

    #155319

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L30 to S, F31 to S

    Promoter

    M13

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTet-SNAP-POC5

    Plasmid

    #136786

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-POC5 (POC5 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid

    #99315

    Purpose

    Negative control for luciferase validation vectors

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    neutral region; chr3: 55198740-55200041
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-mCherry-(F30-2xPepper)10

    Plasmid

    #129401

    Purpose

    mCherry mRNA reporter tagged with (F30-2xPepper)10

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    mCherry-(F30-2xPepper)10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GtCCR2_EYFP_pcDNA3.1

    Plasmid

    #159121

    Purpose

    This plasmid encodes GtCCR2 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

    Insert

    GtCCR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINB-OTULIN (full-length, aa 1-352)

    Plasmid

    #61464

    Purpose

    Expresses human OTULIN (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

    Insert

    OTULIN (OTULIN Human)
    

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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