We narrowed to 8,736 results for: Set

1. pST50Trc1-DHFRCYD

   Plasmid

   #63952

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Covalent Yet Dissociable (CYD) [AGTEFCA]

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST50Trc1-HPCDHFR

   Plasmid

   #63939

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Protein C (HPC)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pimEJ5GFP

   Plasmid

   #44026

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ5GFP egfp-based chromosomal break reporter
   

   Expression

   Mammalian

   Mutation

   pCAGGS promoter and eGFP separated by pgkPURO cas…

   Promoter

   pCAGGS

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAC574

   Plasmid

   #127975

   Purpose

   AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   hygB (hygromycin resistance marker)
   

   Use

   Fungal expression

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans trpC promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BASIC_SEVA_67_pKD46.10

   Plasmid

   #178942

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   March 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS416-PTEF1-MCP-no_intron

   Plasmid

   #127597

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   1xFLAG-MCP expression cassette
   

   Expression

   Yeast

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV GG hUbC-dsRED

   Plasmid

   #84034

   Purpose

   Contains Golden Gate cassette to express up to four gRNAs and dsRED

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDC.109

   Plasmid

   #227189

   Purpose

   T7 IPTG-driven Cas9

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   SpyCas9
   

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FIRESFLIP-PBCS-E670

    Plasmid

    #228701

    Purpose

    Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE2 SENSOR
    

    Use

    Synthetic Biology and Unspecified

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYTK-DN1

    Plasmid

    #180282

    Purpose

    Cas9 cassette (TU1)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS - pPGK1 - Cas9 - tPGK1 - ConR1
    

    Expression

    Bacterial

    Promoter

    pPGK1

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    ermE*/tipA

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPGxRF3

    Plasmid

    #138384

    Purpose

    T2A-GAL4 knock-in cassette

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL4
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6_N7-sgRNA2

    Plasmid

    #190664

    Purpose

    Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    N7 sgRNA2
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPAP004

    Plasmid

    #160911

    Purpose

    E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    lacZ selection cassette
    

    Expression

    Yeast

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSWAP_Entry_H2_beta_CM

    Plasmid

    #184870

    Purpose

    pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    March 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. HHM-iATPSnFR1.0

    Plasmid

    #102546

    Purpose

    expresses ATP sensor in bacteria

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVII

    Plasmid

    #162750

    Purpose

    MSCV backbone

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BASIC_SEVA_5a7_pKD46.10

    Plasmid

    #178937

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. FDP-P10B2-URA3

    Plasmid

    #130875

    Purpose

    Integration cassette for OrthoRep containing mKate driven by promoter 10B2

    Depositor

    Chang Liu

    Article

    Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

    Insert

    mKate
    

    Use

    Expression of mkate, leu2(q180*), and ura3 on ort…

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge