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pFlare9A-Dup34

Plasmid

#90267

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Stoilov et al Proc Natl Acad Sci U S A. 2008 Aug 12;10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSG 25

Plasmid

#63867

Purpose

Expression of beta-galactosidase at high levels in E. coli

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pRSFDuet1_CylLL_CylM

Plasmid

#208759

Purpose

Expresses modified cytolysin L (mCylLL) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLL
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NAP1L1-Halo

Plasmid

#175342

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NAP1L1 (NAP1L1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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T7_CC_PEmax_IVT_Template

Plasmid

#216793

Purpose

Template for in vitro transcription of PEmax.

Depositor

Daniel Bauer

Article

Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

Insert

PEmax

Expression

Mammalian

Promoter

T7

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT7RNAP

Plasmid

#170101

Purpose

expresses T7 RNAP

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

T7-RNA polymerase

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCHW111040

Plasmid

#222451

Purpose

Level 1, Position 3 containing CBS4-CMV::RUBY::OCS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

CBS4-CMV1::RUBY::OCS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor

Plasmid

#25938

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

ClopHensor

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pCHW101008

Plasmid

#222453

Purpose

Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

CBS4-miniDFR::OsL5::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Butelase AY

Plasmid

#221847

Purpose

Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

Val237Ala, Thr238Tyr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33-Gm

Plasmid

#65098

Purpose

bacterial expression vector with Gentamycin selection, based on pBAD33

Depositor

Juan Tomas

Article

Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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mito-EcSTH

Plasmid

#211921

Purpose

Mitochondrial localization of EcSTH

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-As-crRNA

Plasmid

#78956

Purpose

Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTu

Plasmid

#31172

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Tu (tufB E. coli)

Tags

His

Expression

Bacterial

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pSCrhaB2

Plasmid

#113634

Purpose

An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

Depositor

Miguel Valvano

Article

Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

Type

Empty backbone

Expression

Bacterial

Promoter

PrhaB

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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