We narrowed to 18,061 results for: Mut

1. pLentiCRISPR-E

   Plasmid

   #78852

   Purpose

   Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

   Depositor

   Phillip Abbosh

   Article

   Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Cas9-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIneo-lambdaN-HA-HsSmg6_I

   Plasmid

   #146551

   Purpose

   Mammalian Expression of HsSmg6

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsSmg6 (SMG6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T594C, A699G and two non sil…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4-NHK-MYC-His6

   Plasmid

   #185350

   Purpose

   Assessing of ubiquitination in cells

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   a1-antitrypsin Hong Kong Null mutant
   

   Use

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   NHK mutant

   Promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-Rheb-N153T

   Plasmid

   #19997

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Ras homolog enriched in brain 1 - N153T (RHEB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Asparagine 153 to Threonine

   Promoter

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pTBNde(min)

   Plasmid

   #15125

   Depositor

   Franco Pagani

   Article

   Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

   Insert

   alpha-globin EDB fibronectin minigene (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   alpha-globin

   Available Since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pAS_4xMma PylT_FLAG-Mma PylRS AF

   Plasmid

   #140023

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y271A, Y349F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myc-RILPL1

   Plasmid

   #201470

   Purpose

   For overexpression in cultured cells to visualize RILPL1 protein interactions.

   Depositor

   Suzanne Pfeffer

   Article

   Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

   Insert

   RILPL1 (RILPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6691 Bicistronic_ires_puro

   Plasmid

   #64335

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV Myc IRES GFP

    Plasmid

    #18770

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    Myc (Myc Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

11. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid

    #227246

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α and pTRE3G

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdSh.PGK.Cas9

    Plasmid

    #58253

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    Human codon-optimized S. pyogenes Cas9
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmStrawberry-Atg4BC74A

    Plasmid

    #21076

    Depositor

    Tamotsu Yoshimori

    Article

    Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

    Insert

    Atg4B(C74A) (Atg4b Mouse)
    

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    220-222 TGC(Cys) is changed to GCA (Ala)

    Promoter

    Available Since

    June 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. OPEN-A*24:02

    Plasmid

    #235133

    Purpose

    E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. OPEN-HLA-A*01:01

    Plasmid

    #235134

    Purpose

    E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe B-Raf V600E

    Plasmid

    #17544

    Depositor

    Channing Der

    Article

    Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

    Insert

    B-Raf (BRAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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