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We narrowed to 13,043 results for: PAC

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Showing: 5801 - 5820 of 13043 results

  4. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  13. MK01

    Bacterial Strain
    #195090
    Purpose
    Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).
    Depositor
    Sander Tans
    Article
    Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1
    Bacterial Resistance
    Chloramphenicol
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only

  16. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  17. pAdDeltaF6

    Plasmid
    #112867
    Purpose
    AAV helper plasmid
    Depositor
    James M. Wilson
    Insert
    E4, E2a and VA
    Use
    AAV
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112867-plasmid-map-sequence-id-372973

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  18. pMD2.G

    Plasmid
    #12259
    Purpose
    VSV-G envelope expressing plasmid
    Depositor
    Didier Trono
    Insert
    VSV G
    Use
    Lentiviral; Envelope
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12259-plasmid-map-sequence-id-443804

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  19. pRS314-URA3

    Plasmid
    #11004
    Depositor
    Francisco Malagon
    Insert
    URA3 (URA3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Available Since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only
    11004-plasmid-map-sequence-id-6838

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  20. YEplac181-BUD32

    Plasmid
    #10997
    Depositor
    Francisco Malagon
    Insert
    BUD32 (BUD32 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Available Since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    10997-plasmid-map-sequence-id-6832

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Showing: 5801 - 5820 of 13043 results