We narrowed to 9,277 results for: Pol;

1. pEMS1994

   Plasmid

   #49124

   Purpose

   AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple266-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   S100B

   Available Since

   May 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLentiDP1.0

   Plasmid

   #171094

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-Axin1

   Plasmid

   #109370

   Purpose

   Transient over-expression of human Axin1

   Depositor

   Mariann Bienz

   Article

   Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

   Insert

   Axin1 (AXIN1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hGFAP-GFP

   Plasmid

   #40592

   Depositor

   Albee Messing

   Article

   Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

   Insert

   GFP-S65T
   

   Use

   Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Bacterial and Mammalian

   Mutation

   Construct contains a mutated, humanized hGFP(S65T…

   Promoter

   hGFAP promoter

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3-Rac1

   Plasmid

   #92039

   Purpose

   Expresses mEos3-Rac1 fusion protein

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos3-dnRac1

   Plasmid

   #92040

   Purpose

   Expresses mEos3 fusion with dnRac1 (T17N).

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T17N (dominant negative)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3-caRac1

   Plasmid

   #92041

   Purpose

   Expresses mEos3 fusion with caRac1 (Q61L)

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q61L (constitutively active)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4_NFAT-RE-CMVmin-BC0524-luc2

   Plasmid

   #227139

   Purpose

   Barcoded assay, Ca2+ sensor; barcode BC0524

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
   

   Expression

   Mammalian

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2159

    Plasmid

    #70120

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. peGFP C2-IST1

    Plasmid

    #218582

    Purpose

    Expresses GFP-tagged human IST1 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    IST1 (IST1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. W118-1_hTGM2-Flag

    Plasmid

    #180407

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid

    #140892

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE)

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Str-KDEL_SBP-mCherry-CCR1

    Plasmid

    #222311

    Purpose

    Synchronize the trafficking of CCR1 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1 TMEM192-Flag

    Plasmid

    #112742

    Purpose

    express pLJM1 TMEM192-Flag

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    TMEM192-Flag (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. peGFP C3-SPG20-PAAA

    Plasmid

    #218581

    Purpose

    Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Proline 172 to Alanine and Tyrosine 174 t…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDG458 V3

    Plasmid

    #226959

    Purpose

    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-His6-LOXCAT

    Plasmid

    #140084

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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