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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,772 results for: sta

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Showing: 5801 - 5820 of 14772 results

  2. P100 (3OMC)

    Plasmid
    #28159
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SND1 (SND1 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Tudor domain (residues 650-910).
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid
    #105811
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105811-plasmid-map-sequence-id-204856

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  14. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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  15. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid
    #208573
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    d2nucEGFP
    HygroR
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK/CMV and minP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208573-plasmid-map-sequence-id-412948

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  19. mcherry p62 delta UBA silent

    Plasmid
    #187984
    Purpose
    mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572
    Depositor
    Sascha Martens
    Publication
    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755
    Insert
    p62 (NUP62 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lacking UBA domain (aa389-434) but it has the sil…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    187984-plasmid-map-sequence-id-374443

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Showing: 5801 - 5820 of 14772 results