We narrowed to 30,912 results for: LIS

1. pLenti-OC-IRES-BSD

   Plasmid

   #53118

   Purpose

   To create stable cell clones with high-level expression of Cas9 and OCT1

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Inserts

   OCT1 (POU2F1 Human)
   Cas9
   

   Use

   Lentiviral

   Tags

   IRES-BSD, NLS, and P2A

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pET21-pelB-LaG-30

   Plasmid

   #172754

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-30
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

   Plasmid

   #205320

   Purpose

   All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISAba30 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaM-8

   Plasmid

   #172772

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Per2:iLuc

   Plasmid

   #160293

   Purpose

   target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

   Depositor

   Joseph Takahashi

   Article

   Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

   Insert

   Per2LUC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   no promotor

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-CAR-GECO1

   Plasmid

   #45493

   Purpose

   Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   CAR-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuidPuro-hTP73_sgRNA-1

   Plasmid

   #88855

   Purpose

   CRISPR KO of Trp73

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp73
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-HA-UBE3B-IRES-Puro

   Plasmid

   #107345

   Purpose

   Expresses HA tagged UBE3B in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174884

    Purpose

    Targeting vector for generating Sox2 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Sox2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDA093

    Plasmid

    #131390

    Purpose

    generation of Tet-On RPE

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR223-BMRF1(EA-D)

    Plasmid

    #195807

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BMRF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BMRF1 (BMRF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-MXD1

    Plasmid

    #178443

    Purpose

    doxycycline-inducible expression of Human MXD1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    MXD1 (MXD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP788_Traptavidin-EZH2-FL-CO

    Plasmid

    #211781

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-EZH2
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. RCAS-K-ras

    Plasmid

    #11549

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

18. GFP-STAM1

    Plasmid

    #164628

    Purpose

    Expresses STAM1 in mammalian cells with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    STAM1 (STAM Human)
    

    Use

    Tags

    cycle 3 GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ-SpRY-Act3.0

    Plasmid

    #178957

    Purpose

    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR223-EBNALP

    Plasmid

    #195817

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNALP

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNALP (EBNA-LP Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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