We narrowed to 19,002 results for: Tec

1. pGWB555

   Plasmid

   #74885

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK-YEE-BE3

   Plasmid

   #85177

   Purpose

   Expresses YEE-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   YEE-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2355_Tier3(PB)-iRFP_PuroR

   Plasmid

   #169654

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

   Plasmid

   #213402

   Purpose

   douple floxed, myristoylation (myr) site for membrane targeting and EGFP

   Depositor

   Katrin Willig

   Article

   Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

   Insert

   EGFP
   

   Use

   AAV

   Tags

   C-terminal (Ct) cytoplasmic domains of low densit…

   Expression

   Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-eNme2-C.NR

   Plasmid

   #185672

   Purpose

   Expresses nuclease active eNme2-C.NR in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   eNme2-C.NR nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB455

   Plasmid

   #74837

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVenus-VSVG

   Plasmid

   #11914

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Expression

   Mammalian

   Mutation

   F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hRedOpsin-TGFB1

   Plasmid

   #164427

   Purpose

   AAV plasmid expressing TGFB1 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

   Insert

   Tgfb1 (Tgfb1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human red opsin

   Available Since

   Nov. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB404

    Plasmid

    #74798

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY026

    Plasmid

    #84741

    Purpose

    Expresses huAsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC102

    Plasmid

    #118883

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid

    #181783

    Purpose

    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pShuttle-CMV-iRFP

    Plasmid

    #31856

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Adenoviral

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgLenti-orthogonal

    Plasmid

    #105997

    Purpose

    Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; S.pyogenes sgrna expressio…

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB418

    Plasmid

    #74812

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-Puro cTnT FUCCI

    Plasmid

    #136935

    Purpose

    donor plasmid for cardiac-specific FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Troponin T promoter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    cTnT

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK-VRER-BE3

    Plasmid

    #85173

    Purpose

    Expresses VRER-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VRER-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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