We narrowed to 19,562 results for: Tec

Showing: 5821 - 5840 of 19562 results

Results per page:

Sort results by:

pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIV-D3psi/delta env/delta Vif/delta Vpr

Plasmid

#132928

Purpose

Production of SIV-VLPs containing Vpx protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

Gag, pol, and vpx

Use

Lentiviral

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW2657

Plasmid

#252577

Purpose

TRV2 multiplex vector expressing Ymu1-WFR, AtPDS3 gRNA12 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtPDS3 gRNA 12 and AtCHLl1 gRNA4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Article

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlagNICD1

Plasmid

#20183

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 1 Intracellular Domain (Notch1 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

Contains murine Notch 1 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-blueCKAR

Plasmid

#118472

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SaCas9 TadDE

Plasmid

#193846

Purpose

Express TadDE (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SaKKH-ABE8e

Plasmid

#138502

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_734

Plasmid

#216097

Purpose

All-in-one, knockout, delivers Cas9 & gRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCC001

Plasmid

#124451

Purpose

Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HH-BsaI

Expression

Yeast

Mutation

gRNA expression cassette from pUDP002 modified to…

Promoter

TDH3 promoter from S.cerevisiae (already present …

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB578

Plasmid

#141095

Purpose

E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Inserts

Cas9 and tracrRNA
repeat-spacer-repeat array

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CuO-LacI-NLS–SNAPf

Plasmid

#227368

Purpose

Lentiviral expression of LacI–SNAP

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

LacI NLS SNAPf

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge