We narrowed to 34,882 results for: cas

1. pGB411

   Plasmid

   #134760

   Purpose

   pCambia CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBC-MT1T2.2

   Plasmid

   #134751

   Purpose

   PCR template for sgRNA cloning

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   sgRNA-TaU3p
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEC581

   Plasmid

   #174369

   Purpose

   RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   RFP with PAM rich sequence upstream promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23117

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

   Plasmid

   #91861

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) R0179A/K1080A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1080A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2CT-His-MBP-Lbu_C2c2_E299A

   Plasmid

   #91855

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) E299A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   E299A

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIBR002

   Plasmid

   #177665

   Purpose

   SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZmU3-gRNA

   Plasmid

   #53061

   Purpose

   Transcription of guide RNA (gRNA) in maize cells

   Depositor

   Caixia Gao

   Article

   Liang et al J Genet Genomics. 2014 Feb 20;41(2):63-8

   Insert

   zmU3 promoter - gRNA
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   maize U3

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Lbu_C2c2_K1087A

   Plasmid

   #91864

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K1087A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1087A

   Promoter

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. hs-calx1

   Plasmid

   #1740

   Depositor

   Craig Montell

   Article

   Wang et al Neuron 2005 Feb 3;45(3):367-78.

   Insert

   calx1 (Calx Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   N/A

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. hs-calx2

    Plasmid

    #1741

    Depositor

    Craig Montell

    Article

    Wang et al Neuron 2005 Feb 3;45(3):367-78.

    Insert

    calx2 (Calx Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    N/A

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pSIBR005

    Plasmid

    #177668

    Purpose

    Constitutive expression of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIBR001

    Plasmid

    #177628

    Purpose

    SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2CT-His-MBP-Lbu_C2c2_R1072A

    Plasmid

    #91859

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) R1072A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    R1072A

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2-AcrIIA4

    Plasmid

    #174034

    Purpose

    In vitro transcription of anti-CRISPR AcrIIA4 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    AcrIIA4
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-AcrIIA2

    Plasmid

    #174033

    Purpose

    In vitro transcription of anti-CRISPR AcrIIA2 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    AcrIIA2
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2CT-His-MBP-Lbu_C2c2_K1082A

    Plasmid

    #91863

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) K1082A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1082A

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPOR1CB0113

    Plasmid

    #117552

    Purpose

    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPOR2KN0079

    Plasmid

    #117646

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPOR0SP0011

    Plasmid

    #117530

    Purpose

    Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-xCas9 3.7 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTBL2670 MTK3-PEmax

    Plasmid

    #226663

    Purpose

    MTK3 part containing the coding sequence of PEmax

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    PEmax
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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